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At1g79490 SYNGENTA 70615

Genomic Sequence (from TAIR)

aagataattgatagttgatgagcaaaacatttaaagaaatttgtactaaattgatgagttaatttttttttgttttttgtttttgatgtgtgtgtggtta
attggttattttttctctaacgttaaaatgattttttgctaattggtaatgatttttctctactattgtaatctaaattaaagctgcacttgttgtaaaa
acgttttcttttgaatctaatttaacatgaaattcaaaaaaaataatctattttgggaaaaccccacactgttctaaagcctctggttttagggtttaag
ccgacggaggagacggaaaaatcgccatctctggagtccaggttagaattgattcctcaaatttcaattcttcgcattacatgacccaataattccgtct
acatcttcgATGATCCGTGGAAGAACCGCGAAAGTAATTCCCAGAAATGTCATTTTTACGCTTAGTAGGAGTTCCATTTCTGAGTCTCCATTGATTTCTC
CGAGTCGAATAAACCCTAAATTAGCCGGTAGTTTCTCGTTCAACATCCGATTATTGAGCTATTTCACTGTTAGAAATGGCTTTTGTCCCGATTGTTCTGT
ACCGAGAGACCCTAATTTTGTTGGATTGACTACACAATGTCGGAGTATTGTCAGGAGGTTTTGTAGTGAAAAGATTGGAAGTAGTGAATCTAGTGGCTGG
ACAGAGGAGGTTGAGTATTTAGATGAGTCGGGTAGTGTACTACACAGTGGTAAGGGAATAAGATCAGTAGAGCCAGGGCTTGATGACCATGTAATGGTTG
GTGGATTGAAGAAGCCTTACATGAATGCTTCTTCTGTTGCAAAGATTGTTGAAGTTGTTCAGAGGTGGAAATGGGGACCGGAGTTGGAGACTCAGTTAGA
TAAACTCCAGTTTGTTCCGAATATGGTTCATATTACGCAGTCTTTGAAGATTGTTAAAGAGGTTGATGCGGCGCTGAGTCTGTTTAGGTGGGCTAAGAAG
CAGCCTTGGTATCTGCCTTCGGATGAATGTTATGTCGTGTTGTTTGATGGGTTGAACCAGGGAAGGGATTTTGTTGGGATTCAGTCGTTGTTTGAGGAGA
TGGTTCAAGACTCGAGTAGTCACGGTGATTTGTCGTTTAACGCCTATAACCAAGTGATTCAGTATTTGGCTAAAGCTGAGAAACTGGAGGTTGCTTTTTG
TTGTTTCAAAAAGGCTCAAGAGTCGGGATGCAAAATTGATACGCAGACATATAATAATCTAATGATGTTGTTTCTGAACAAAGGTCTCCCGTATAAGGCA
TTTGAGATTTATGAGAGCATGGAGAAAACTGATAGTTTGTTGGATGGGTCAACTTATGAGCTGATAATTCCAAGCTTGGCCAAATCCGGCCGTCTTGATG
CAGCTTTCAAGCTTTTTCAGCAAATGAAAGAAAGGAAACTCCGACCAAGCTTTAGTGTGTTTTCTTCGCTTGTTGATTCAATGGGGAAAGCTGGTCGATT
GGACACATCAATGAAGGTTTACATGGAAATGCAGGGATTTGGCCATAGGCCATCAGCAACTATGTTTGTTTCCTTGAT◄TGATTCATATGCTAAAGCTGG
TAAGCTGGATACTGCTCTTAGGCTTTGGGATGAGATGAAGAAGTCAGGTTTCAGGCCAAACTTTGGATTGTACACAATGATCATTGAATCTCATGCAAAA
TCAGGAAAGCTTGAAGTAGCAATGACGGTTTTCAAAGACATGGAGAAAGCTGGGTTTTTACCGA◄CACCATCCACATATTCGTGTCTATTGGAGATGCAT
GCTGGATCTGGGCAAGTAGACTCTGCAATGAAAATCTATAACTCCATGACTAATGCTGGGTTAAGGCCTGGCCTGAGCAGTTATATTTCTCTTCTTACAC
TTCTGGCCAACAAAAGACTTGTCGATGTTGCTGGGAAGATACTACTCGAGATGAAAGCAATGGGGTATTCCGTAGATGTCTGCGCTAGCGATGTTTTGAT
GATATATATCAAAGATGCTTCTGTGGATCTTGCTCTGAAATGGCTTAGGTTCATGGGTTCTTCAGGGATCAAAACAAACAATTTTATCATCAGGCAGTTA
TTTGAATCATGCATGAAAAATGGTCTATACGATTCAGCTAGGCCTTTGCTGGAGACACTCGTGCATTCTGCTGGAAAAGTTGACTTGGTGCTTTACACTT
CGATTCTCGCCCATCTTGTCAGATGCCAAGACGAAGATAAAGAGAGACAATTGATGTCAATCCTCAGCGCTACCAAGCATAAAGCTCATGCCTTTATGTG
TGGTCTCTTCACAGGTCCAGAACAGAGGAAACAACCAGTTCTAACGTTTGTCAGAGAGTTTTACCAAGGGATTGATTACGAACTCGAAGAAGGAGCTGCT
AGGTACTTTGTGAATGTCCTTCTCAACTACCTTGTCTTGATGGGTCAAATAAACCGAGCTCGATGTGTCTGGAAAGTAGCGTACGAGAACAAACTCTTTC
CAAAAGCCATCGTCTTTGATCAACACATTGCTTGGTCTCTCGATGTGAGAAACTTATCTGTCGGAGCTGCGCTCATAGCCGTGGTTCACACTCTCCACAG
GTTCAGAAAACGAATGCTTTACTACGGAGTAGTCCCGAGACGTATAAAGCTAGTCACAGGACCGACATTGAAGATTGTAATTGCTCAAATGCTGAGCTCT
GTTGAGTCGCCTTTTGAAGTCAGCAAAGTCGTCTTGAGGGCACCAGGAGAGTTGGTGATGGAGTGGTTCAAGAAACCGATCGTGCAACAGTTTCTTCTGA
ACGAGATCCCATCACGGTCTGATATATTGATGCATAAGATGAACGTGATGTTCCCAAGCTCGGCTCCTGAGCTTAGATCTATGTCACCTCCCAAACCACT
CATGTCATC►GAAAGCGTTCTAAcaggggagacaaaagtcatgggtttttgtaggcgggttctctgtttcagatctctcattgttaatacttgtctgaaa
atataatggaaatgatatttacgtctttaaatcattgttagttcatttttgttcgattacgtctttaatgtctccgagtaaagcttacgtccttatcctg
gataggaattaatctgtcccgagtattcctacgttcttatcctggataggaattaatctgtccatgtatccaaacatcacctctcagttaatatctccaa
tttacacgacgcgctaaaggtgagaggagatctcacttgccttcttcactcactctgcagcaacttcgagatcaagttaaaggtcagattccttaattct
ccatttcttatttgcttcgttgcagagaagaattaggttttcccgatcttcggattctgattctgattttcttttgcgattattggcttggcgataagaA
TGG

Border_1 (70615_AbB)


TGCGGCTCGGTACGGAAGTTGACCGTGCTTGTCTCGATGTAGTGGTTGACGATGGTGCAGACCGCCGGCATGTCCGCCTCGGTGGCACGGCGGATGTCGG
CCGGGCGTCGTTCTGGGCTCATGGATCCACGTGTGGAAGATATGAATTTTTTTGAGAAACTAGATAAGATTAATGAATATCGGTGTTTTGGTTTTTTCTT
GTGGCCGTCTTTGTTTATATTGAGATTTTTCAAATCAGTGCGCAAGACGTGACGTAAGTATCCGAGTCAGTTTTTATTTTTCTACTAATTTGGTCGTTTA
TTTCGGCGTGTAGGACATGGCAACCGGGCCTGAATTTCGCGGGTATTCTGTTTCTATTCCAACTTTTTCTTGATCCGCAGCCATTAACGACTTTTGAATA
GATACGCTGACACGCCAAGCCTCGCTAGTCAAAAGTGTACCAAACAACGCTTTACAGCAAGAACGGAATGCGCGTGACGCTCGCGGTGACGCCATTTCGC
CTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATACAC
CAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATGTAT
CAAGGAAACAAACATAGTAATTGATGGCCTATGGCATTTCCATGTAAACCTTCA

Start of BLAST Alignment:	NT 1354 / Exon 1 of 1
First 15 bp Perfect Match:	NT 1169 / Exon 1 of 1

BLAST Details:
Query Sequence: 70615_AbB 
Length: (754 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At1g79490 68414.t08471 pentatricopeptide (PPR) repeat-con...   194  0.92      1
At4g26910 68417.t03598 2-oxoacid dehydrogenase family pro...   193  0.95      1


>At1g79490 68414.t08471 pentatricopeptide (PPR) repeat-containing protein low
           similarity to fertility restorer [Petunia x hybrida] GI:22128587;
           contains Pfam profile PF01535: PPR repeat
        Length = 2731

  Minus Strand HSPs:

 Score = 194 (35.2 bits), Expect = 2.6, P = 0.92
 Identities = 148/247 (59%), Positives = 148/247 (59%), Strand = Minus / Plus

Query:   746 TACATGGAAATG-CCATAGGCCATCAATTACTATGTTTGTTTCCTTGATACATATTGTGG 688
             | || |||  || |||||||||||||   ||||||||||||||||||||  || |  |  
Sbjct:  1279 TGCAGGGATTTGGCCATAGGCCATCAGCAACTATGTTTGTTTCCTTGATTGAT-TCATA- 1336

Query:   687 TGTAAACAAATTG-ACGCTTAGACAACTTAA-TAACACATTGCGGACGTTTTTAAT--GT 632
             ||  || |  | | | |||  || | ||    | |  | ||| ||| |   | ||   ||
Sbjct:  1337 TGCTAA-AGCTGGTAAGCTG-GATA-CTGCTCTTAGGCTTTG-GGATGAGATGAAGAAGT 1392

Query:   631 ACTGAATTAACGCCGAA-TT-GAATTCGATTTGGTGTATCGA-GATTGGTTATG-AAATT 576
              | |  || | ||| || || | |||  |     || |||   || |  | ||| ||| |
Sbjct:  1393 -CAGGTTTCAGGCCAAACTTTGGATTGTACACAATG-ATCATTGAATC-TCATGCAAAAT 1449

Query:   575 CAG--ATGCTAGT-GTAATGTATTGGTAATTTGGGAAGATATAATAGGAAGCAAGGCTAT 519
             |||  | ||| |  |||  | | ||    |||   |||| ||   || ||||  || | |
Sbjct:  1450 CAGGAAAGCTTGAAGTA--GCAATGACGGTTTTCAAAGACATGG-AGAAAGCTGGGTT-T 1505

Query:   518 TTATCCA 512
             ||| | |
Sbjct:  1506 TTACCGA 1512


>At4g26910 68417.t03598 2-oxoacid dehydrogenase family protein similar to
           SP|P36957 Dihydrolipoamide succinyltransferase component of
           2-oxoglutarate dehydrogenase complex, mitochondrial precursor (EC
           2.3.1.61) {Homo sapiens}; contains Pfam profiles PF00198: 2-oxo acid
           dehydrogenases acyltransferase (catalytic domain), PF00364:
           Biotin-requiring enzyme
        Length = 3404

  Plus Strand HSPs:

 Score = 193 (35.0 bits), Expect = 3.0, P = 0.95
 Identities = 117/197 (59%), Positives = 117/197 (59%), Strand = Plus / Plus

Border_2 (70615_LB)


GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAATATATCCCCTCGCTGAAAGCGTTCTAACAGGGGAGAC
AAAAGTCATGGGTTTTTGTAGGC:GGGTTCTCTGTTTCAGATCTCTCATTGTTAATACTTGTCTGAAAATATAATGGAAATGATATTTACAG:TCTTTAA
ATCATTGTT::AGTTCATTTTTGTTCGACTTA:CGTCTTTAATGTC::TCCGAGTA:A:AGCTTACG:TC:CTTATCCTGGATAGGAATTAATCTGTCCC
GAGTATTCCTACGTTCTTATCCTGGATAGGAATTAATCTGTCCATGTATCCAAACATCACCTCTCAGTTAATATCTCCAATTTACACGACGCGCTAAAGG
TGAGAGGAGATCTCACTTGCCTTCTTCACTCACTCTGCAGCAACTTCGAGATCAAGTTAAAGGTCAGATTCCTTAATTCTCCATTTCTTATTTGCTTCGT
TGCAGAGAAGAATTAGGTTTTCCCGATCTTCGGATTCTGATTCTGATTTTCTTTTGCGATTATTGGCTTGGCGATAAGAATGGC:GGCAACATCACTATT
GTACAATCAGCTCAAGGTTTGAGTCTCGCCTTTTTGTTTTCAGAATGTTGAATGTTGGCTTCTTACGGTAAAATCGAGTTTGATTCTAATACGGTATATC
GTGTATAGGCTGCTGACAYCATTCTTCTTGNTGGCTGGTCCAAATGTGATTGAATCCGAGGAACATATTCTTCGAATGGCC

Start of BLAST Alignment:	NT 2498 / Exon 1 of 1
First 15 bp Perfect Match:	NT 2498 / Exon 1 of 1

BLAST Details:
Query Sequence: 70615_LB Border #2 
Length: (869 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

T8K14 51297                                                   3384  1.1e-146  1
F3O9 51361                                                     457  1.4e-12   1
T17D12 51056                                                   230  0.14      1
T1P17 67111                                                    203  0.94      1


T8K14 51297
        Length = 78,574

  Minus Strand HSPs:

 Score = 3384 (513.8 bits), Expect = 1.1e-146, P = 1.1e-146
 Identities = 685/691 (99%), Positives = 686/691 (99%), Strand = Minus / Plus

Query:   868 GCCATTCGAAGAATATGTTCCTCGGATTCAATCACATTTGGACCAGCCANCAAGAAGAAT 809
             ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct: 34187 GCCATTCGAAGAATATGTTCCTCGGATTCAATCACATTTGGACCAGCCAACAAGAAGAAT 34246

Query:   808 GRTGTCAGCAGCCTATACACGATATACCGTATTAGAATCAAACTCGATTTTACCGTAAGA 749
             |+| ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
Sbjct: 34247 GGT-TCAGCAGCCTATACACGATATAC-GTATTAGAATCAAACTCGATTTTACCGTAAGA 34304

Query:   748 AGCCAACATTCAACATTCTGAAAACAAAAAGGCGAGACTCAAACCTTGAGCTGATTGTAC 689
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 34305 AGCCAACATTCAACATTCTGAAAACAAAAAGGCGAGACTCAAACCTTGAGCTGATTGTAC 34364

Query:   688 AATAGTGATGTTGCCGCCATTCTTATCGCCAAGCCAATAATCGCAAAAGAAAATCAGAAT 629
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 34365 AATAGTGATGTTGCCGCCATTCTTATCGCCAAGCCAATAATCGCAAAAGAAAATCAGAAT 34424

Query:   628 CAGAATCCGAAGATCGGGAAAACCTAATTCTTCTCTGCAACGAAGCAAATAAGAAATGGA 569
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 34425 CAGAATCCGAAGATCGGGAAAACCTAATTCTTCTCTGCAACGAAGCAAATAAGAAATGGA 34484

Query:   568 GAATTAAGGAATCTGACCTTTAACTTGATCTCGAAGTTGCTGCAGAGTGAGTGAAGAAGG 509
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 34485 GAATTAAGGAATCTGACCTTTAACTTGATCTCGAAGTTGCTGCAGAGTGAGTGAAGAAGG 34544

Query:   508 CAAGTGAGATCTCCTCTCACCTTTAGCGCGTCGTGTAAATTGGAGATATTAACTGAGAGG 449
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 34545 CAAGTGAGATCTCCTCTCACCTTTAGCGCGTCGTGTAAATTGGAGATATTAACTGAGAGG 34604

Query:   448 TGATGTTTGGATACATGGACAGATTAATTCCTATCCAGGATAAGAACGTAGGAATACTCG 389
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 34605 TGATGTTTGGATACATGGACAGATTAATTCCTATCCAGGATAAGAACGTAGGAATACTCG 34664

Query:   388 GGACAGATTAATTCCTATCCAGGATAAGGACGTAAGCTTTACTCGGAGACATTAAAGACG 329
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 34665 GGACAGATTAATTCCTATCCAGGATAAGGACGTAAGCTTTACTCGGAGACATTAAAGACG 34724

Query:   328 TAAGTCGAACAAAAATGAACTAACAATGATTTAAAGACTGTAAATATCATTTCCATTATA 269
             ||| |||||||||||||||||||||||||||||||||| |||||||||||||||||||||
Sbjct: 34725 TAA-TCGAACAAAAATGAACTAACAATGATTTAAAGAC-GTAAATATCATTTCCATTATA 34782

Query:   268 TTTTCAGACAAGTATTAACAATGAGAGATCTGAAACAGAGAACCCGCCTACAAAAACCCA 209
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 34783 TTTTCAGACAAGTATTAACAATGAGAGATCTGAAACAGAGAACCCGCCTACAAAAACCCA 34842

Query:   208 TGACTTTTGTCTCCCCTGTTAGAACGCTTTC 178
             |||||||||||||||||||||||||||||||
Sbjct: 34843 TGACTTTTGTCTCCCCTGTTAGAACGCTTTC 34873


F3O9 51361
        Length = 114,498

  Minus Strand HSPs:

 Score = 457 (74.6 bits), Expect = 1.4e-12, P = 1.4e-12
 Identities = 176/261 (67%), Positives = 177/261 (67%), Strand = Minus / Plus