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At5g12840 SYNGENTA 70913

Genomic Sequence (from TAIR)
acttaatatcaaaaaccacaattctggcaaaaaagaaaaatccccaaaatctagggttttcttctcgcccaatttcacttttcttctacgaaattct
ccattcctgccggctgtcgggttttctgaatcgattctccttcaccaacttcttctctggttctgttcgattctgattttttttcaaggtcaatttttt
cttctctttaaactctgcaaaatcgtgatcgattaaattcacctcagggttttttgatttctgaaagaagttaatcttcttcgaaggcgattgcaaaaga
gtgctctgctgtgaatttccactggtatgttcaattttttctgtttttgaaattttgtttgctagaattcgtgaagggattgaaaaaattgatgcaggtg
gctaaagaatctcttgaatattacgagcttatgtaggtttagagtgtaaattcgagacatgtgagattttggtgctttctcatatttttgcatgcttctg
cattgcttcatagccctaaaattgctctcttttttttgtagttggagtctgtatctttttcacggttttagtgatgaatttttttttttgattggatttt
gaattggttactgaacatgtgttgacttgattatgtagagATGCAATCAAAACCGGGAAGAGAAAACGAAGAGGAAGTCAATAATCACCATGCTGTTCAG
CAGCCGATGATGTATGCAGAGCCCTGGTGGAAAAACAACTCCTTTGGTGTTGTACCTCAAGCGAGACCTTCTGGAATTCCATCAAATTCCTCTTCTTTGG
ATTGCCCCAATGGTTCCGAGTCAAACGATGTTCATTCAGCATCTGAAGACGGTGCGTTGAATGGTGAAAACGATGGCACTTGGAAGGATTCACAAGCTGC
AACTTCCTCTCGTTCAGgtttgagttcttggtctttaggcctacttggtgtattgttcatattcatcatttctgcagtactgttttactggatgacctag
tgatcactgtcgaaagtgatgttgttccacgttcggaatgttgcaacttggtgaagagttgttcctcaagcaggcagggaaattaaatgggcatttgtgt
gattcggcactatagggcgtgtcaagttcatgaatctggtccttgaaatatacttgcattgtaattgtagggtttcatagttctatccatgccaaggaga
ttctgtctcgttgatatgtgcattgtgatttctttattttgtttgtaatggtgtctttcttcaacttcagTAGATAATCACGGAATGGAAGGAAATGACC
CAGCGCTCTCTATCCGTAACATGCATGATCAGCCACTTGTACAACCACCAGAGCTTGTTGGACACTATATCgtatgtggtttcctctcattccttaatat
atatatatatatgctttatgagttcttgagctgttaaattgacttcttattggtccctcaaaaggatttatcttgtataacgcccttttcatatgtatga
taaactcaaaacttccttacagGCTTGTGTCCCAAACCCATATCAGGATCCATATTATGGGGGATTGATGGGAGCATATGGTCATCAGCAATTGgtatct
tgtcgaaacctctttcaatctctacaatagttccatatggtgcagtgtttatttgatgtatgtgcgcttttgccactaagtacccgtttgtggtgatttt
atttactgttggaatgtgtctgggttcagcaatagtcagtgagatgcttttaaagttcatttctgaaataactgagaatccaaagttatatctgaaatta
tcgagaacaactaatggggattcttggggtcttataagttatatgtaaagttttcgatgatctgagtctatttgttgcatttattttgttcattggttta
aacagGGTTTTCGTCCATATCTTGGAATGCCTCGTGAAAGAACAGCTCTGCCACTTGACATGGCACAAGAGCCCGTTTATGTGAATGCAAAGCAGTACGA
GGGAATTCTAAGGCGAAGAAAAGCACGTGCCAAGGCAGAGCTAGAGAGGAAAGTCATCCGGGACAGAAAGgtatttttttctcatttatgaaaattgtac
ttgattggtgtcctggtaatctaaggatggtgtagagtccataatagaagtggacccatgctatatgaaggattgataatgagcattaagaatttgcaag
tttggtttgtcgctcggtaaacttgtattgactatgtttgaaatgcgacagCCATATCTTCACGAGTCAAGACACAAGCATGCAATGAGAAGGGCACGAG
CGAGTGGAGGCCGGTTTGCGAAGAAAAGTGAGGTAGAAGCGGGAGAGGATGCAGGAGGGAGAGACAGAGAAAGGGGTTCAGCAACCAACTCATCAGGCTC
TGAACAAGTTGAGACAGACTCTAATGAGACCCTGAATTCTTCTGGTGCACCATAAtaaaaaaagccaaagctctgagaggagagagagacacacactttg
gctaatataatcattgcctcaaaccggcaaatcattcttggctttttcgtttttgtgtttgctagttgttcttgtcagagtctcatattgtgtgggttta
acagttatgatgaatgtacaaagagcgagttatgttaggtgttagattttggagacaagagactaaggaatagcaagtaggtcttgtttttattctttga
ccttttttttctcttttgcaaaattgaaaaatacgtttgcttaatcgtttgcctttctgaccacaaagtgttaacaaggctataacaatgcttcagattt
gtagtcaaattctctgttttcgccaacttaaacattatttgaccggaggagagtgattagaaagtagaagaaacctattaaacattatgttttgcaaaga
aaaaaaaaacagcttgcaataagttgcatagtttgctcatggaagtttcaagtttgcatataaaccagatttgaaaaggacagtgaaaaaaaagaagttg
ttaa

Border_1 (70913_LB2U)

AATCNATTCATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGTGTACATTAAATGCTGATATACACCCTAGATTT
TGGGGATTTTTCTTTTTTGCCAGAATTGTGGTTTTTGATATTAAGNAATTTATTGAAAAATCGAAATAGAGTAAGTTATATGATGATGATGAATTTACAC
AAAAGACCGAGATTTGATAAGATCACNTTACGAAAGTGAAAAATCTTTTTGCCACGTGGCTGCTCTATGAAAGATGCCAGCGCGCGCAGATCTGGGATCT
TCTCCGGCTGACGTTATCATAGGTTCATTTTTAATTTGTTTTGTTTTTTCTTATATACTAGAAAGAAATTAAATTTCTTTTAAAAAACAGGAGAAATTTG
GGTCCAAGNGACACGCAAGTGATGGGCTGGGACGAGGGGACACCTGTAAAATGTGGGGACCCTATTGGCAAAAACAATTAAAAGATTTTTTTTTGTTAAA
NAAATAAGTTAATTAGTTCAATATTGGTAATTTNTTTNTTTNACAAAATAANATAAAGNGTTTNCCCTTCATNAAAAAAATATAGTTGTTTTAAGAAAAT
AATAATANTACTCCANCTGAAAAAANTNTTTTCCATAAATAATNGGNGGTAAAAAAAAAACCTTGGAAAGGTTTTTAAAANTTTTTTAAAGGGNTNCNAA
AGGGAAAANNCCGTNCGAANGGTTAAAAATTNGGGGGTTNTCNCCGGAATTAAAGGNCNCCTNACNNCNNA

Start of BLAST Alignment:< 508 / Exon 1 of 7 (Intron 1,2 in 5’ UTR)
First 15 bp Perfect Match:< 579 / Exon 1 of 7 (Intron 1,2 in 5’ UTR)
BLAST Details:
Query Sequence: 70913_LB2U 
Length: (771 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g12840 68418.t01338 CCAAT-binding transcription factor...   252  0.0037    1
At3g46480 68416.t04683 oxidoreductase, 2OG-Fe(II) oxygena...   185  0.997     1


>At5g12840 68418.t01338 CCAAT-binding transcription factor (CBF-B/NF-YA) family
           protein contains Pfam profile: PF02045 CCAAT-binding transcription
           factor (CBF-B/NF-YA) subunit B
        Length = 2898

  Minus Strand HSPs:

 Score = 252 (43.9 bits), Expect = 0.0037, P = 0.0037
 Identities = 84/120 (70%), Positives = 84/120 (70%), Strand = Minus / Plus

Query:   137 CAAAAACCACAATTCTGGCAAAAAAGAAAAATCCCCAAAATCTAGGGTGTATATCAGCAT 78
             |||||||||||||||||||||||||||||||||||||||||||||||| |   ||  |  
Sbjct:     1 CAAAAACCACAATTCTGGCAAAAAAGAAAAATCCCCAAAATCTAGGGTTTTCTTCT-CGC 59

Query:    77 TTAATGT-ACACTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTA--CTGAAT 21
               ||| | ||  ||   | ||  |||    |||| | | ||  | |   ||   ||||||
Sbjct:    60 CCAATTTCACTTTTCTTCTACGAAATTCTCCATTCCTGCCGGCTGTCGGGTTTTCTGAAT 119


>At3g46480 68416.t04683 oxidoreductase, 2OG-Fe(II) oxygenase family protein low
           similarity to gibberellin 20-oxidase [gi:4678370]; contains Pfam
           domain PF03171, 2OG-Fe(II) oxygenase superfamily
        Length = 2422

  Plus Strand HSPs:

 Score = 185 (33.8 bits), Expect = 5.8, P = 0.997
 Identities = 107/168 (63%), Positives = 107/168 (63%), Strand = Plus / Plus


Border_2 (70913_LB1D)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCG:ATT:AATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCATATATCCCCAATTTCACTTTTCTTCTACGA
AATTCTCCATTCCTGCCGGCTGTCGGGTTTTCTGAATCGATTCTCCTTCACCAACTTCTTCTCTGGTTCTGTTCGATTCTGATTTTTTTTCAAGGTCAAT
TTTTTCTTCTCTTTAAACTCTGCAAAATCGTGATCGATTAAATTCACCTCAGGGTTTTTTGATTTCTGAAAGAAGTTAATCTTCTTCGAAGGCGATTGCA
AAAGAGTGCTCTGCTGTGAATTTCCACTGGTATGTTCAATTTTTTCTGTTTTTGAAATTTTGTTTGCTAGAATTCGTGAAGGGATTGAAAAAATTGATGC
AGGTGGCTAAAGAATCTCTTGAATATTACGAGCTTATGTAGGTTTAGAGTGTAAATTCGAGACATGTGAGATTTTGGTGCTTTCTCATATTTTTGCATGC
TTCTGCATTGCTTCATAGCCCTAAAATTGCTCTCTTTTTTTTGTAGTTGGAGTCTGTATCTTTTTCACGGTTTTAGNGATGAATTTTTTTTTTGNANGGA
NTTTGAANTGGNNNCCGNANNNGGGGTGGCCTGGTTTTNGAAANGAGCCATCNAAACCGGGAANNAAAAACCGAGAGGGAGGCATTATCCCCCTTNCTNT
CNACNACCCANTATTGTTNCNAAACCCCTNGGGGAAAAACNACCTCTTTGGGGGTGGANCCCAAGGNAAACCCTT

Start of BLAST Alignment:< 617 / Exon 1 of 7 (Intron 1,2 in 5’ UTR)
First 15 bp Perfect Match:< 569 / Exon 1 of 7 (Intron 1,2 in 5’ UTR)
BLAST Details:
Query Sequence: 70913_LB1D 
Length: (873 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g12840 68418.t01338 CCAAT-binding transcription factor...  2731  4.1e-119  1


>At5g12840 68418.t01338 CCAAT-binding transcription factor (CBF-B/NF-YA) family
           protein contains Pfam profile: PF02045 CCAAT-binding transcription
           factor (CBF-B/NF-YA) subunit B
        Length = 2898

  Plus Strand HSPs:

 Score = 2731 (415.8 bits), Expect = 4.1e-119, P = 4.1e-119
 Identities = 631/752 (83%), Positives = 631/752 (83%), Strand = Plus / Plus

Query:   124 AATTCAGTACATTAA-AAACGTCCGCAATGTGTTATTAAGTTGTCATATATC-CCCAATT 181
             ||||| |   |  || |||  ||| |||  | | |    ||| || | | || |||||||
Sbjct:    11 AATTCTGGCAAAAAAGAAAAATCCCCAAAATCT-AGG--GTTTTC-T-TCTCGCCCAATT 65

Query:   182 TCACTTTTCTTCTACGAAATTCTCCATTCCTGCCGGCTGTCGGGTTTTCTGAATCGATTC 241
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:    66 TCACTTTTCTTCTACGAAATTCTCCATTCCTGCCGGCTGTCGGGTTTTCTGAATCGATTC 125

Query:   242 TCCTTCACCAACTTCTTCTCTGGTTCTGTTCGATTCTGANNNNNNNNCAAGGTCAATTTT 301
             |||||||||||||||||||||||||||||||||||||||        |||||||||||||
Sbjct:   126 TCCTTCACCAACTTCTTCTCTGGTTCTGTTCGATTCTGATTTTTTTTCAAGGTCAATTTT 185

Query:   302 TTCTTCTCTTTAAACTCTGCAAAATCGTGATCGATTAAATTCACCTCAGGGTTTTTTGAT 361
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   186 TTCTTCTCTTTAAACTCTGCAAAATCGTGATCGATTAAATTCACCTCAGGGTTTTTTGAT 245

Query:   362 TTCTGAAAGAAGTTAATCTTCTTCGAAGGCGATTGCAAAAGAGTGCTCTGCTGTGAATTT 421
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   246 TTCTGAAAGAAGTTAATCTTCTTCGAAGGCGATTGCAAAAGAGTGCTCTGCTGTGAATTT 305

Query:   422 CCACTGGTATGTTCAATTTTTTCTGTTTTTGAAATTTTGTTTGCTAGAATTCGTGAAGGG 481
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   306 CCACTGGTATGTTCAATTTTTTCTGTTTTTGAAATTTTGTTTGCTAGAATTCGTGAAGGG 365

Query:   482 ATTGAAAAAATTGATGCAGGTGGCTAAAGAATCTCTTGAATATTACGAGCTTATGTAGGT 541
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   366 ATTGAAAAAATTGATGCAGGTGGCTAAAGAATCTCTTGAATATTACGAGCTTATGTAGGT 425

Query:   542 TTAGAGTGTAAATTCGAGACATGTGAGATTTTGGTGCTTTCTCATATTTTTGCATGCTTC 601
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   426 TTAGAGTGTAAATTCGAGACATGTGAGATTTTGGTGCTTTCTCATATTTTTGCATGCTTC 485

Query:   602 TGCATTGCTTCATAGCCCTAAAATTGCTCTCNNNNNNNNGTAGTTGGAGTCTGTATCTTT 661
             |||||||||||||||||||||||||||||||        |||||||||||||||||||||
Sbjct:   486 TGCATTGCTTCATAGCCCTAAAATTGCTCTCTTTTTTTTGTAGTTGGAGTCTGTATCTTT 545

Query:   662 TTCACGGTTTTAGNGATGAANNNNNNNNNNGNAN-GGANTTTGAANTGGNNNCCGNANNN 720
             ||||||||||||| ||||||            |  ||| |||||| |||   | | |   
Sbjct:   546 TTCACGGTTTTAGTGATGAATTTTTTTTTTTGATTGGATTTTGAATTGGTTACTGAACAT 605

Query:   721 GGGGTGGCCTGGTTTTNGAAANGAGCCATCNAAACCGGGAANNAAAAACCGAGAGGGAGG 780
             | | || | || || |  | |   || ||| ||||||||||   |||||  ||||| || 
Sbjct:   606 GTGTTGACTTGATTATGTAGAGATGCAATCAAAACCGGGAAGAGAAAACGAAGAGGAAGT 665

Query:   781 CATTATCCCCCTTNCTNTCNACNACCCANTATTGTTNCNAAACCCCTNGGGGAAAAACNA 840
             || ||  | || | || |  |  | ||  |  |||    | | |||| | |||||||| |
Sbjct:   666 CAATAATCACCATGCTGTTCAGCAGCCGATGATGTATGCAGAGCCCTGGTGGAAAAACAA 725

Query:   841 CCTCTTTGGGGGTGGANCCCAAGGNAAACCCT 872
             |  |||||| | || | | ||||  | ||| |
Sbjct:   726 CTCCTTTGGTGTTGTACCTCAAGCGAGACCTT 757