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At1g22090 SYNGENTA 71067

Genomic Sequence (from TAIR)
aagaaaaaaaaaagaagtcaaaatcaaacttatgaatgaaagtaaagctcagaataaacggaaagtttccgtggttggaatgtgtaaacccttaccggct
tgttgtcggaatctcgagaaatgctcctgtgttgctcggacgatcagtgtgtgtacttttgctgaagtgaaagattatgaaaagatctggggtaaatcag
taaatgtaaaagcccttacctaaagcgatggcgttttaagggtacggtgaaaaaacaataaaaattgaacatgtgcgttgcaacatcgtcaagattgaca
attgaagtcccaaatgaagcccatgaatgaaaccttcgccgccttcgccagacaaaacctaaaactataaaagcagcagtcgctaatttttggaagg
agaaggagaaagATGAGTCGCCGGGTGGAATACACGTTAGCTCCTCCGCAGAGGAATGAATCTGACgtaattctctcatctttttatattgtttagcttg
tgtactagtgttcctttcttacgatgttaagggtagtgattgattgattgattattttctctgatgggtcttagGGTTTTGATTATCCCGACGGTATCCC
AATATCATACAATCTTCATAGGCTTCGTCACTTTGAGTGTGAAGGCAGTTATCCCAAGTATCCTTATGGTTCTTTGGTCAAGTTTTATGCAATGGTGGGA
CTTCATCGTTACAATGTGTTGGAGgtaaacaatatagtgcttttctagtcgatatgctactggaactattaattattattatgatataccaattacattc
tcaatctctttttccatctgtcagGGGAAAAATTTGCAGCTCGATACCCTAAAGAGTTTCAACATGAGAATCAATTGTGGTGCTTCTTCTTACTACATTA
CTTTGGCTGCACGCGTTCCAGATAGCGGTTTGAAGCAGATCTTTCAGGTTCTAGTTCATGAAGAGCGTCTTGGCAGTTTAGACATGACATGTACTATCGC
TAGACCTCGAGgtaaacatgtaactttacttgatcagtcattttgacttgtggcagtttacctgtacatctaacacttgctggttaatattgcacagTGA
CTACCAATGTGCCTTTTCTACGTCCGCACAGCGAATCAGAGTATGATTATATGGACAATGATGAATTGCCTGACTGGCCTTCAGAGATTGCTTTCGATGA
TACAAAACGGTTTCATCTGgtaagattattatttataaagtacatcctttgttgtttgtttaatgaattatcatatggatttataaatcatttaaaatgc
atgaatcaatagagacacttgtggttttgcttttgcctactagttaattttattgagataccttgcagGTGAAGGAATCAGAGTTGCGAGACAATGATTG
GATTCGACTCTATTTGGAACTTACACTTGTTGCTCACGATAGGTTTCTTACAGTTgtaagtgtcttttctctctttcatttgtttgcatatcaaaatgat
ttagtggcattcaatattggtaataattaacattacatgattttgtttatgcagCACTATCTCTCCCAGTTGGAGATTGTGAAAGTTGCGATTGAAGAAG
TGGAGCAACCGAATGCGAGTCTCAACACCAAAACTACATTTGTCTACATAACTTATAAGGACTTGGCAAAGGCTCAGATTGGTGAGCCGGTTGATCGCAA
AGCTATTGTTAGAAAAATCATCAATGAGACTACGGGACTCTTGAGACTCCGGGGTGATTATTGGAGTGGAGAAAGAAGTGTGATCACTCCGGAGGAGGAA
TATATGCTTCTCCATGGCGGAGAAAAAGTTCGAAACAATGAGCAGCGTTCTAAAAAACTTAAGCGTCGTGTAGGTGTTCATAGGCTATGGCGATGGTGGT
ACCAGGCTTACAAAAACCGTGGCCTCCGCTCGTCGTCTTATTAAgtaatgaatgtggataagctgcataactattattaatggtctctgactaagttatt
ttgtgcgtttggactgtttatgtggatttgtttttttgcggtttgtgtaagttataagggtgtactgttctcaacatctgaacactaatgtttagtttaa
tcgtgcttggttttgtcttcttggaccaaatcctagttgtgaaatttgcctttactgcttcttctctgctttagttttctcttcatctctattcttttcc
atggttttggcagatgatgttataaaaaataagattagagggaaatacttttaaccaaaaatagacattataaccataggaaacaagactagttaaacct
gggaagagaaagagaccattgatgaggaattggcaaagcagagagacaatgtcttaaagcacattttgctctgtttcctctaatcataattccagctact
tca

Border_1 (71067_LB1D)

TCTCGATACACCAAATCGAATTCATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTGTCAATCTTGA
CGATGTTGCAACGCACATGTTCAATTTTTATTGTTTTTTCACCGTACCCTTAAAACGCCATCGCTTTAGGTAAGGGCTTTTACATTTACTGATTTACCCC
AGATCTTTTCATAATCTTTCACTTCAGCAAAAGTACACACACTGATCGTCCGAGCAACACAGGAGCATTTCTCGAGATTCCGACAACAAGCCGGTAAGGG
TTTACACATTCCAACCACGGAAACTTTCCGTTTATTCTGAGCTTTACTTTCATTCATAAGTTTGATTTTGACTTCTTTTTTTTTCTTTGGCTACCCAAAA
AGATCSATNGGTTTTTNNATCTTTGATTTCATGTAGAGCTAAAAGAACAGGAACNACCACSCCRAGAGGTACNYACATTTATGGGYCGWRTTTTGAGACT
TTAGYACCGGAGGGCANGGGATTATTATCCGMSACCCTGGGACNAAAAAATGATTNNNGGANAANNNCAAAGTNTGCTANTTGACCGNTTTTTTTGCTGG
CCCNCTTTTTGCGNGCTGGAAGGGGATTTTTCCNGAAATTNANTNCATNTNGNGCCGTTCAGGANAAAAAATGNGAGGNGNTTTTTTTTACTACNTTACC
TTGGGAAGNCGCCTTTAAAAAAAAAGGTTTTTTTCGGAACAAAAACAAACTAGGGGTTGNGGATCTTTAAAANCTAACTATTGGGCACNTA

Start of BLAST Alignment:< 102 / Upstream
First 15 bp Perfect Match:< 104 / Upstream
BLAST Details:
Query Sequence: 71067_LB1D 
Length: (791 letters) 
Database: GenBank AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

gb|AC069252.4|AC069252 Genomic sequence for Arabidopsis t...  2255  1.0e-95   1


>gb|AC069252.4 Genomic sequence for Arabidopsis thaliana BAC F2E2 from
            chromosome I
        Length = 91,825

  Minus Strand HSPs:

 Score = 2255 (344.4 bits), Expect = 1.0e-95, P = 1.0e-95
 Identities = 559/714 (78%), Positives = 569/714 (79%), Strand = Minus / Plus

Query:   788 GTGCCCAATAGTTA-GNTTTTAAAGAT-CCNCAACCCCTAGTTTGTTT-TTGTTCCGAAA 732
             ||| |  ||||||| | |||| ||||  ||  |||| ||| |  || | |||   |  ||
Sbjct: 62285 GTGACACATAGTTAAGTTTTTTAAGAAACCGAAACCACTACTA-GTCTATTGACACAGAA 62343

Query:   731 AAAACCNNNNNNNNNAAAGGCGNCTTCCCAAG-GTAANGTAGTNNNNNNNNNCNCCTCNC 673
             | |||          | ||||    ||  ||| | ||  ||           |   |  |
Sbjct: 62344 AGAACTTTTACTTTTAGAGGCAATCTCA-AAGTGAAACATAC-AAAAAAAATCAGAT-GC 62400

Query:   672 ATTTTTTNTC-CTGAACGGCNCNANATGNANTNAATTTCNGGAAAAATCCCCTTCCAGCN 614
             || ||      |  ||||     | ||  | | |||| |  | | |||   | | |||  
Sbjct: 62401 ATATTCAAAGTCAAAACGTACAGAAATAAAGTAAATT-CAAGTAGAATAGACATTCAGAC 62459

Query:   613 CGCAAAAAGNGGG-CCAGCAAAAAAANCGGTCAANTAGCANACTTTGNNNTTNTCCNNNA 555
              |||||  | ||| |||||||||||| |   ||| ||||| || |||    | | |   |
Sbjct: 62460 AGCAAAT-GAGGGACCAGCAAAAAAAACAA-CAACTAGCAAACATTGGACATATACATAA 62517

Query:   554 ATCATTTTTTNGTCCCAGGGTSKCGGATAATAATCCCNTGCCCTCCGGTRCTAAAGTCTC 495
             |||||||||| |||||| |||++|||||||||||||| |||||| | ||+||| |||| |
Sbjct: 62518 ATCATTTTTTAGTCCCATGGTCTCGGATAATAATCCCATGCCCTACAGTACTAGAGTCAC 62577

Query:   494 AAAAYWCGRCCCATAAATGTRNGTACCTCTYGGSGTGGTNGTTCCTGTTCTTTTAGCTCT 435
             || |++||+|||||||||||+ ||||||||+||+|| || ||||||||||| ||||||||
Sbjct: 62578 AAGATACGACCCATAAATGTATGTACCTCTTGGCGTCGTTGTTCCTGTTCTCTTAGCTCT 62637

Query:   434 ACATGAAATCAAAGATNNAAAAACCNATSGATCTTTTTGGGTAGCCAAAGNNNNNNNNN- 376
             | ||||||||| |||   | ||||| | +|| |||||| | |||||||||          
Sbjct: 62638 AGATGAAATCAGAGACTGAGAAACCTAGCGAACTTTTTCG-TAGCCAAAGAAAAAAAAAA 62696

Query:   375 GAAGTCAAAATCAAACTTATGAATGAAAGTAAAGCTCAGAATAAACGGAAAGTTTCCGTG 316
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 62697 GAAGTCAAAATCAAACTTATGAATGAAAGTAAAGCTCAGAATAAACGGAAAGTTTCCGTG 62756

Query:   315 GTTGGAATGTGTAAACCCTTACCGGCTTGTTGTCGGAATCTCGAGAAATGCTCCTGTGTT 256
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 62757 GTTGGAATGTGTAAACCCTTACCGGCTTGTTGTCGGAATCTCGAGAAATGCTCCTGTGTT 62816

Query:   255 GCTCGGACGATCAGTGTGTGTACTTTTGCTGAAGTGAAAGATTATGAAAAGATCTGGGGT 196
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 62817 GCTCGGACGATCAGTGTGTGTACTTTTGCTGAAGTGAAAGATTATGAAAAGATCTGGGGT 62876

Query:   195 AAATCAGTAAATGTAAAAGCCCTTACCTAAAGCGATGGCGTTTTAAGGGTACGGTGAAAA 136
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 62877 AAATCAGTAAATGTAAAAGCCCTTACCTAAAGCGATGGCGTTTTAAGGGTACGGTGAAAA 62936

Query:   135 AACAATAAAAATTGAACATGTGCGTTGCAACATCGTCAAGATTGACAATTGACG 82
             |||||||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct: 62937 AACAATAAAAATTGAACATGTGCGTTGCAACATCGTCAAGATTGACAATTGAAG 62990


Border_2 (71067_LB2U)

AGAGAAGCACCNCAATTGATTCTCATGTTGNANCCTCTTAGGGTATCGAGCTGCAAATTTTTCCCCTGACAGATGGAAAAAGAGATTGAGAATGTAATTG
GTATATCATAATAATANNTAATAGTTCCAGTAGCATATCNACTANNAAAGCACTATATTGTTTACCNCCANCNCATTGTANCGATGAAGTCCCCACCATT
GCATAAAACNTGACCAAAGAACCATAAGGATNCTTGGGNTAACTNCCTTCTCNCTCAAAGTGACGAAGCCTANGAAGATTGTATGATATTGGGATACCGT
CGGGATAATCAAAACCCTAAGNNCCATCAGAGAAAATAATCAATCAATCAATCANTACCCTTANCNTCGTGAAGAAGGAACNCTAGTACACAAGCTAAAC
AATATAAAAAGATGAGAGAATTACGTCAGATTCATTCCTCTGCGGAGGAGCTAACGTGTATTCCACCCGGCGACTCATCTTTCTCCTTCTCCTTCCAAAA
ATTAGCGACTGCTGCTTTTATAGTTTTAGGTTTTGTCTGGCGAAGGCGGCGAAGGTTTCATTCATGGGCTTCATTCA

Start of BLAST Alignment:< 97 / Upstream
First 15 bp Perfect Match:< 97 / Upstream
Reverse Complement
BLAST Details:
Query Sequence: 71067_LB2U Border #2 
Length: (577 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

F2E2 61356                                                    2623  2.6e-112  1
F28L5 68136                                                    398  8.5e-12   1
T23E23 51237                                                   333  7.4e-09   1



F2E2 61356
        Length = 91,825

  Minus Strand HSPs:

 Score = 2623 (399.6 bits), Expect = 2.6e-112, P = 2.6e-112
 Identities = 545/575 (94%), Positives = 545/575 (94%), Strand = Minus / Plus

Query:   575 AATGAAGCCCATGAATGAAACCTTCGCCGCCTTCGCCAGACAAAACCTAAAACTATAAAA 516
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 62996 AATGAAGCCCATGAATGAAACCTTCGCCGCCTTCGCCAGACAAAACCTAAAACTATAAAA 63055

Query:   515 GCAGCAGTCGCTAATTTTTGGAAGGAGAAGGAGAAAGATGAGTCGCCGGGTGGAATACAC 456
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 63056 GCAGCAGTCGCTAATTTTTGGAAGGAGAAGGAGAAAGATGAGTCGCCGGGTGGAATACAC 63115

Query:   455 GTTAGCTCCTCCGCAGAGGAATGAATCTGACGTAATTCTCTCATCTTTTTATATTGTTTA 396
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 63116 GTTAGCTCCTCCGCAGAGGAATGAATCTGACGTAATTCTCTCATCTTTTTATATTGTTTA 63175

Query:   395 GCTTGTGTACTAGNGTTCCTT-CTTCACGANGNTAAGGGTANTGATTGATTGATTGATTA 337
             ||||||||||||| ||||||| ||| |||| | |||||||| ||||||||||||||||||
Sbjct: 63176 GCTTGTGTACTAGTGTTCCTTTCTT-ACGATGTTAAGGGTAGTGATTGATTGATTGATTA 63234

Query:   336 TTTTCTCTGATGGNNCTTAGGGTTTTGATTATCCCGACGGTATCCCAATATCATACAATC 277
             |||||||||||||  |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 63235 TTTTCTCTGATGGGTCTTAGGGTTTTGATTATCCCGACGGTATCCCAATATCATACAATC 63294

Query:   276 TTCNTAGGCTTCGTCACTTTGAGNGAGAAGGNAGTTANCCCAAGNATCCTTATGGTTCTT 217
             ||| ||||||||||||||||||| | ||||| ||||| |||||| |||||||||||||||
Sbjct: 63295 TTCATAGGCTTCGTCACTTTGAGTGTGAAGGCAGTTATCCCAAGTATCCTTATGGTTCTT 63354

Query:   216 TGGTCANGTTTTATGCAATGGTGGGGACTTCATCGNTACAATGNGNTGGNGGTAAACAAT 157
             |||||| |||||||||||||||||| ||||||||| ||||||| | ||| ||||||||||
Sbjct: 63355 TGGTCAAGTTTTATGCAATGGTGGG-ACTTCATCGTTACAATGTGTTGGAGGTAAACAAT 63413

Query:   156 ATAGTGCTTTNNTAGTNGATATGCTACTGGAACTATTANNTATTATTATGATATACCAAT 97
             ||||||||||  |||| |||||||||||||||||||||  ||||||||||||||||||||
Sbjct: 63414 ATAGTGCTTTTCTAGTCGATATGCTACTGGAACTATTAATTATTATTATGATATACCAAT 63473

Query:    96 TACATTCTCAATCTCTTTTTCCATCTGTCAGGGGAAAAATTTGCAGCTCGATACCCTAAG 37
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
Sbjct: 63474 TACATTCTCAATCTCTTTTTCCATCTGTCAGGGGAAAAATTTGCAGCTCGATACCCTAA- 63532

Query:    36 AG-GNTNCAACATGAGAATCAATTGNGGTGCTTCT 3
             || | | |||||||||||||||||| |||||||||
Sbjct: 63533 AGAGTTTCAACATGAGAATCAATTGTGGTGCTTCT 63567


F28L5 68136
        Length = 48,404

  Plus Strand HSPs:

 Score = 398 (65.8 bits), Expect = 8.5e-12, P = 8.5e-12
 Identities = 220/364 (60%), Positives = 220/364 (60%), Strand = Plus / Plus