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At2g45330 SYNGENTA 7724

Genomic Sequence (from TAIR)

ttcaacgcaatcgccgatcaaacgaatccgcggaggaggactgactactgaccgcctgaaaaaaactaaaccgggaagaacccaaactacgtgacgttta
tttgaactgtcatcgaatatcatgccgtctcgtcattcacttatctgagtgggctttataatagaaaggcccagtacataatgtatatcgattcctaagt
tggcgaatacataatgttgctgcgtctctgtcgtcatcgacttcctcttcctctgactttgagcagctctgtcttcttctcgaaatcgtctcctgtttct
tctgctttcATGGATGCTTCAAATCCCAATTCTTCTAGAAAATCTAATGTCTCTTCCTTCGCTCAGTCCAGTCGAAGgtgaattctaatttttcaaatta
ttcgaaaagtcaaaatcccttttgattttccctcgttttttcatgtaaggtttggtgtttgattttgatggatttagCGGTGGTAGAGGAGGAGGATATG
AGAGAGATAACGATCGACGGAGACCTCAGGGTCGTGGCGACGGTGGAGGCGGAAAGGATAGAATCGATGCACTTGGACGACTCTTgtaagtgtaatcgat
caatactggattctagatgttagagaattagcttgaattttgaatctataatggattgataatcagGACGAGAATATTGCGACATATGGCTACTGAGCTG
AGATTGAACATGAGAGGTGATGGTTTTGTTAAAGTTGAAGATTTACTTAACCTGAATTTGAAAACTTCTGCAAATATTCAGTTAAAGTCACACACGATTG
ATGAAATTAGAGAGgttagtttcatttttttcacttgtttttgtcaacgagcattttcgattggagacgtttttgagatgagtttgtgtatggttttcgt
taacttgcagGCTGTGAGAAGGGACAATAAGCAACGGTTTAGTCTCATCGATGAGAATGGAGAGCTCTTGATTCGCGCTAACCAAGGCCATTCGATCACG
gtaatagaacttgaacaatgaatccttagcatttggctgttttcttcttcgttgagacacagtagcactatgattggatttttgttctcctagttttgat
caatagctttagcttatgtctttgtgcgcagACGGTTGAGTCAGAGAAGTTACTTAAACCAATACTGTCACCAGAAGAAGCTCCAGgtactactaggaca
catacttgtaaaattatgttattgttatgtgttactcgagttcatttgaatttctgtaatggcagTGTGTGTACATGGAACTTATAGGAAGAATTTGGAA
TCCATCTTAGCATCGGGCTTAAAGCGTATGAATAGAATGCATGT◄TCACTTCTCTTG►TGGATT►ACCAACAGATGGTGAAGTGATTAGTGgtaatattt
ga◄ttaatcctattgttgttggtcagacattatttcatatgcatatttatctgattttccatatcgacataatttggctaagGCATGAGAAGAAATGTAA
ATGTTATCATCTTCCTCGACATCAAGAAAGCTCTTGAAGgtatgagaacttaataacttgtctgaaaaaatagtgatatgcccttgaaagattcaatatt
aaaattccactttattggtgcacccagATGGGATTGCGTTCTACATATCAGACAACAAAGTGATTTTGACTGAAGGCATTGATGGTGTATTGCCTGTCGA
TTACTTCCAGAAGATCGAGTCTTGGCCTGATCGGCAATCCATACCTTTCTGAttcatataattcaacatcatgcgaagattgacaggatcctatgacaat
gattgtgaggattcttctgaaccttgattatgtaatgttgtctcagtgttttcaattgcacatatgacaatttatgaaaactttcaagattatgttgttt
cctttgcccaaagacatgagaagtgcagaaataaatcaactgttaataaaaagcgttttatggttcttaaataatttaaggccctaaagcccaaggcccc
aaac

Border_1 (7724_LB2D)


CAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACATGCATTCTATTCA
TACGCTTTAAGCCCGATGCTAAGATGGATTCCAAATTCTTCCTATAAGTTCCATGTACACACACTGCCATTACAGAAATTCAAATGAACTCGAGTAACAC
ATAACAATAACATAATTTTACAAGTATGTGTCCTAGTAGTACCTGGAGCTTCTTNTGGNGACAGTATTGGTTTAAGTAACTTCTCTGACTCAACCGTCTG
CGCACAAAGACATAAGCTAAAGCTATTGATCAAAACTAGGAGAACAAAAATCCAATCATAGTGCTACTGTGTCTCAACGAAGAAGAAAACAGCCAAATGC
TAAGGATTCATTGTTCAAGTTCTATTACCGTGATCGAATGGCCTTGGTTAGCGCGAATCAAGAGCTCTCCATTCTCATCGATGAGACTAAACCGTTGCTT
ATTGTCCCTTCTCACAGCCTGCAAGTTAACGAAAACCATACACAAACTCATCTCAAAAACGTCTCCAATCGAAAATGCTCGTTGACAAAAACAAGTGAAA
AAAATGAAACTAACCTCTCTAATTTCATCAATCGTGTGTGACTTTAACTGAATATTTGCAGAAGTTTTCAAATTCAGGTTAAGTAAATCTTCAACTTTAA
CAAAACCATCACCTCTCATGTTCAAT

Start of BLAST Alignment:	NT 1090 / Intron 6 of 7
First 15 bp Perfect Match:	NT 1035 / Exon 6 of 8

BLAST Details:
Query Sequence: 7724_LB2D 
Length: (726 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At2g45330 68415.t05159 tRNA 2'phosphotransferase, putativ...  3204  3.2e-140  1
At5g23600 68418.t02511 tRNA 2'phosphotransferase, putativ...   541  4.8e-36   2
At2g39190 68415.t04432 ABC1 family protein contains Pfam ...   226  0.076     1
At1g51960 68414.t05371 calmodulin-binding family protein ...   180  0.99993   1


>At2g45330 68415.t05159 tRNA 2'phosphotransferase, putative contains similarity
           to Swiss-Prot:Q12272 tRNA 2'phosphotransferase [Saccharomyces
           cerevisiae]; contains Pfam domain PF01885: RNA
           2'-phosphotransferase, Tpt1 / KptA family
        Length = 1659

  Minus Strand HSPs:

 Score = 3204 (486.8 bits), Expect = 3.2e-140, P = 3.2e-140
 Identities = 676/706 (95%), Positives = 676/706 (95%), Strand = Minus / Plus

Query:   726 ATTGAACATGAGAGGTGATGGTTTTGTTAAAGTTGAAGATTTACTTAACCTGAATTTGAA 667
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   453 ATTGAACATGAGAGGTGATGGTTTTGTTAAAGTTGAAGATTTACTTAACCTGAATTTGAA 512

Query:   666 AACTTCTGCAAATATTCAGTTAAAGTCACACACGATTGATGAAATTAGAGAGGTTAGTTT 607
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   513 AACTTCTGCAAATATTCAGTTAAAGTCACACACGATTGATGAAATTAGAGAGGTTAGTTT 572

Query:   606 CATTTTTTTCACTTGTTTTTGTCAACGAGCATTTTCGATTGGAGACGTTTTTGAGATGAG 547
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   573 CATTTTTTTCACTTGTTTTTGTCAACGAGCATTTTCGATTGGAGACGTTTTTGAGATGAG 632

Query:   546 TTTGTGTATGGTTTTCGTTAACTTGCAGGCTGTGAGAAGGGACAATAAGCAACGGTTTAG 487
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   633 TTTGTGTATGGTTTTCGTTAACTTGCAGGCTGTGAGAAGGGACAATAAGCAACGGTTTAG 692

Query:   486 TCTCATCGATGAGAATGGAGAGCTCTTGATTCGCGCTAACCAAGGCCATTCGATCACGGT 427
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   693 TCTCATCGATGAGAATGGAGAGCTCTTGATTCGCGCTAACCAAGGCCATTCGATCACGGT 752

Query:   426 AATAGAACTTGAACAATGAATCCTTAGCATTTGGCTGTTTTCTTCTTCGTTGAGACACAG 367
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   753 AATAGAACTTGAACAATGAATCCTTAGCATTTGGCTGTTTTCTTCTTCGTTGAGACACAG 812

Query:   366 TAGCACTATGATTGGATTTTTGTTCTCCTAGTTTTGATCAATAGCTTTAGCTTATGTCTT 307
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   813 TAGCACTATGATTGGATTTTTGTTCTCCTAGTTTTGATCAATAGCTTTAGCTTATGTCTT 872

Query:   306 TGTGCGCAGACGGTTGAGTCAGAGAAGTTACTTAAACCAATACTGTCNCCANAAGAAGCT 247
             ||||||||||||||||||||||||||||||||||||||||||||||| ||| ||||||||
Sbjct:   873 TGTGCGCAGACGGTTGAGTCAGAGAAGTTACTTAAACCAATACTGTCACCAGAAGAAGCT 932

Query:   246 CCAGGTACTACTAGGACACATACTTGTAAAATTATGTTATTGTTATGTGTTACTCGAGTT 187
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   933 CCAGGTACTACTAGGACACATACTTGTAAAATTATGTTATTGTTATGTGTTACTCGAGTT 992

Query:   186 CATTTGAATTTCTGTAATGGCAGTGTGTGTACATGGAACTTATAGGAAGAATTTGGAATC 127
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   993 CATTTGAATTTCTGTAATGGCAGTGTGTGTACATGGAACTTATAGGAAGAATTTGGAATC 1052

Query:   126 CATCTTAGCATCGGGCTTAAAGCGTATGAATAGAATGCATGTAAACAAATTGACGCTTAG 67
             ||||||||||||||||||||||||||||||||||||||||||  ||   ||  | ||| |
Sbjct:  1053 CATCTTAGCATCGGGCTTAAAGCGTATGAATAGAATGCATGTTCAC---TT--CTCTT-G 1106

Query:    66 ACAACTTAATAACACATTGCGGACGTTTTTAATG-TACTGAATTAA 22
                | |||  |||| || | | | ||  ||| || || | | || |
Sbjct:  1107 TGGA-TTACCAACAGATGGTGAA-GTGATTAGTGGTAAT-ATTTGA 1149


>At5g23600 68418.t02511 tRNA 2'phosphotransferase, putative contains similarity
           to Swiss-Prot:Q12272 tRNA 2'phosphotransferase [Saccharomyces
           cerevisiae]; contains Pfam domain PF01885: RNA
           2'-phosphotransferase, Tpt1 / KptA family
        Length = 639

  Minus Strand HSPs:

 Score = 541 (87.2 bits), Expect = 4.8e-36, Sum P(2) = 4.8e-36
 Identities = 115/122 (94%), Positives = 115/122 (94%), Strand = Minus / Plus

Border_2 (7724_LB1U)


CCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTCACCAACAGATGGTGA
AGTGATTAGTGGTAATATTTGATTAATCCTATTGTTGTTGGTCAGACATTATTTCATATGCATATTTATCTGATTTTCCATATCGACATAATTTGGCTAA
GGCATGAGAAGAAATGTAAATGTTATCATCTTCCTCGACATCAAGAAAGCTCTTGAAGGTATGAGAACTTAATAACTTGTCTGAAAAAATAGTGATATGC
CCTTGAAAGATTCAATATTAAAATTCCACTTTATTGGTGCACCCAGATGGGATTGCGTTCTACATATCAGACAACAAAGTGATTTTGACTGAAGGCATTG
ATGGTGTATTGCCTGTCGATTACTTCCAGAAGATCGAGTCTTGGCCTGATCGGCAATCCATACCTTTCTGATTCATCTCACT

Start of BLAST Alignment:	NT 1047 / Exon 6 of 8
First 15 bp Perfect Match:	NT 1053 / Exon 6 of 8

BLAST Details:
Query Sequence: 7724_LB1U 
Length: (482 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At2g45330 68415.t05159 tRNA 2'phosphotransferase, putativ...  1961  4.4e-84   1
At5g23600 68418.t02511 tRNA 2'phosphotransferase, putativ...   599  2.1e-26   2
At5g54620 68418.t06314 ankyrin repeat family protein cont...   214  0.077     1
At5g24680 68418.t02644 expressed protein similar to unkno...   191  0.71      1


>At2g45330 68415.t05159 tRNA 2'phosphotransferase, putative contains similarity
           to Swiss-Prot:Q12272 tRNA 2'phosphotransferase [Saccharomyces
           cerevisiae]; contains Pfam domain PF01885: RNA
           2'-phosphotransferase, Tpt1 / KptA family
        Length = 1659

  Plus Strand HSPs:

 Score = 1961 (300.3 bits), Expect = 4.4e-84, P = 4.4e-84
 Identities = 397/403 (98%), Positives = 397/403 (98%), Strand = Plus / Plus

Query:    80 TTGTTCACCAACAGATGGTGAAGTGATTAGTGGTAATATTTGATTAATCCTATTGTTGTT 139
             | | | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1107 TGGATTACCAACAGATGGTGAAGTGATTAGTGGTAATATTTGATTAATCCTATTGTTGTT 1166

Query:   140 GGTCAGACATTATTTCATATGCATATTTATCTGATTTTCCATATCGACATAATTTGGCTA 199
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1167 GGTCAGACATTATTTCATATGCATATTTATCTGATTTTCCATATCGACATAATTTGGCTA 1226

Query:   200 AGGCATGAGAAGAAATGTAAATGTTATCATCTTCCTCGACATCAAGAAAGCTCTTGAAGG 259
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1227 AGGCATGAGAAGAAATGTAAATGTTATCATCTTCCTCGACATCAAGAAAGCTCTTGAAGG 1286

Query:   260 TATGAGAACTTAATAACTTGTCTGAAAAAATAGTGATATGCCCTTGAAAGATTCAATATT 319
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1287 TATGAGAACTTAATAACTTGTCTGAAAAAATAGTGATATGCCCTTGAAAGATTCAATATT 1346

Query:   320 AAAATTCCACTTTATTGGTGCACCCAGATGGGATTGCGTTCTACATATCAGACAACAAAG 379
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1347 AAAATTCCACTTTATTGGTGCACCCAGATGGGATTGCGTTCTACATATCAGACAACAAAG 1406

Query:   380 TGATTTTGACTGAAGGCATTGATGGTGTATTGCCTGTCGATTACTTCCAGAAGATCGAGT 439
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1407 TGATTTTGACTGAAGGCATTGATGGTGTATTGCCTGTCGATTACTTCCAGAAGATCGAGT 1466

Query:   440 CTTGGCCTGATCGGCAATCCATACCTTTCTGATTCATCTCACT 482
             ||||||||||||||||||||||||||||||||||||| | | |
Sbjct:  1467 CTTGGCCTGATCGGCAATCCATACCTTTCTGATTCATATAATT 1509


>At5g23600 68418.t02511 tRNA 2'phosphotransferase, putative contains similarity
           to Swiss-Prot:Q12272 tRNA 2'phosphotransferase [Saccharomyces
           cerevisiae]; contains Pfam domain PF01885: RNA
           2'-phosphotransferase, Tpt1 / KptA family
        Length = 639

  Plus Strand HSPs:

 Score = 599 (95.9 bits), Expect = 2.1e-26, Sum P(2) = 2.1e-26
 Identities = 123/127 (96%), Positives = 123/127 (96%), Strand = Plus / Plus