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At2g31530 SYNGENTA 78584

Genomic Sequence (from TAIR)
TCTCAGCGCCGCGTTTTTTCTCTCCTCATCCTCTCGATGAATTCCTCTCAAGCTTGCTTCTTCCATttttctcttagaccaatctcgctttcgcatccct
cctatgccttcctctcgaaacgtaagttttccttccccaaattaagtgcttgggaatcgaaattttaagagattgttgttacctagactagttcaggata
caatcgttagagtttcgttgcttcgaattgattaaaccctaatttcaaaagcacagacctgggtttttcactgttcctgcaaagttgataactttttcac
tgatactatctcttagactagttctcttaacccggaaattctctcaaagtatccaactttatgaacagaaacaattgttgaggcATGTGTTTTGACCTTG
ATAATGTTTTTGCAGGAGACCCATTTCTGTGTTCTCAACCCAGAAAATGCCTTACTACGAATTTGAATATGTCAAGAACGAGACAAGGGCATTCTATACA
AATGAACAGAAGACATCTTCTTATGAAAGAAAGGAAGAGTTTTTCGATAAATTATTCCGATAAGTTCAGAGATGATTCTATGAGTAGCGAGGAGATGCAT
ACAGATGCTTTGGATGTGGAGATTATTCCGCCAGATTCACAGGACATTCGTAATTCACAGAACTCTGCTGTCTCCAACACACTACAGGATGATAGGCCAA
AGTCTTTTAGAAATAGGTTTCTTGATTTTGTTCGGATAAGTTCAGTACTCAATACTGCAGCAGAAAGATTCTTTAAGAGCGAGATAAGGAGGAGGTTGTT
TGTAACGGCTGTTTTGCTTGTACTTAGCCGTGTTGGATATTTTATACCTTTACCTGGATTCGACAGAAGATTAATACCTCAAGATTACCTTAGCTTTGTC
TCGGGTTCTGTTGGTGAGTGTTCTCATgtcatttgagtcttatagtcccttgtttggttgacatttctatgtatttgtagttaacattttatgtttttga
tagaggaactgggtgagTTTGGAGCAGAGATCAAGCTTTCGCTCTTCCAGCTTGGGCTCAGTCCACAAATAATAGCATCCATAATTATGCAGgttaagat
ctctcctcgatttgatgtgtttgcctgagtctgtgcgttctttatatattaagattatgattgatgataaagtgctcatgaaacaccagGTGCTTTGTCA
CGTTCTTCCATCCTTGGTGAAACTGCGAAAGGAAGGCTTAGATGGTCACGAGAAGATCAAGAGTTATATGTGTgtttagtttcttacctctcaagccgtg
agatgaattcttaacatacaattactttgtttgtcttgtgacttttttgcttcattaaacaaagttactaaatcgcctttttggtcttattttattagAT
GGTGGCTATCGTTTTTCTTCGCTATAGTGGAAGCATTAGTGGTGGCTTACACTTCTCTTCAATATTCAGTTTTTGCAGCCACTGCCCAgtaagttaatta
ggattcttcacgttcggggtatcaaaatggtgcttttaaattctatcatccattttgccacaactgatgctaaagtaataatgcatgcttattcagggtc
aagCATGTGATGATGACATCATCTCTGCTAGTCTGTGGTGCAATGACAATGACGTGGCTCTGTGATACAATATCAGAATCTGGATTTGgtatttgattct
ctatatctctatctctataaccattggtagcttaaagtgatgctgatatggagtaattggtggagcttgctcataacctgtttctaagccttttacagGT
CATGGCTCATCTCTGATTATTTGTGTGGGAATATTGACGGGCTACACAGAGACTTTACACAAGATGCTCAATCAAATCTCAGgtacctttggtttttctt
actccacatttctcaaacttatggttagtctttacattagacctgcatgtcttccacatttcctggaggcaactatcattcgcctatcttctcaattgat
ttataaaaaaaggaagcgttcttctcttttgcagGAAGTTTCTCGAACTGGTTGCCTTACTTGTTAGGCTTGCTGGGCATTTTTACCGTAGTAACTATGT
TCGCTGTTGTTGTTACTGAGGGCTGCAGGAAGATTAAACTACAATATTATGGTTTTAAACTTGCTTCTGCTTCGAGgtgatatatgtagctcagcatcat
tattttatcgaaaagattgttgtataattgccacttttaccaatatagctaggagtagattacggttaaagtacatttggatgcaacgtgtggtttactc
atatttgtgtttatgctatgttttgccatttgggcagAGAAGGTTCTCCAATAACAGAGGTGGAGCCGTACATACCTTTTAACATCAACCCGGCTGGAAT
GCAACCTGTTCTCACAACCACATATCTTTTGGCATTCCCCAGCATTCTTGCAAGgtaaattgcttgaagaacctcttactattagggtttaaatcattcc
tgtttcttacttctttgtgttattgttaccatctaaggctgtctaataaggtaatttattgtttttagTATTCTGGGTTCACCTTTCTTGCTAAACATGA
AGGAGATATTAAACCCTGAAAGCACAGTTGGAGCACCACCTTGGGTCTATTACTCAATATATGCGTTTTTCGTCTTTCTCTTCAACATATTTGACATTg
tgagtaaatccattacactttttctcagtctgaatttcagtcttccaatgaacattaaacattacatttaccgggtttcttgttttgttatgtagGCC
AACTTGCCAAAGGAAATAGCGGATTATTTAAACAAAATGGGGGCGAGAATACCGAACATAAAGCCTGGGAAAGCCACAATCGAGTACTTAACGAAGATAC
AAGCATCAACTAGGTTTTGGGgtaagttaagctgtttcattttagatatattgcgtatatggtacaagtataatctctaaaaaccatttcatttggtttg
atgcagGTGGCCTTCTGTTGAGCTTTTTGGCAACAGCTTCTACTGTACTTGACCACTATCTACGCAGCATCAATCAAGGCTTTTCTATCGGATTTACATC
TGTTTTGATCATTgtaagtgttctttccacgtatcaatatcattcagtttagcagtattgatgtcgttgtgttaataatctctattgtttgtgtacagGT
TGGTTCCATTATTGAACTCAGAAGATCTTACCATGCCTACAATGTAATGCCGAGTTTAAGCAAAGCGTTAAAGAGATATGGCGTATAAagggagattgga
gaaacagaagaacttatagatatattattgtcgtttaaactttagaagtttctgtagctcttttgattagtagattaggcattagataatttgtattaag
aaccaaaacacatttccatcataatattagaagacttaaaacccaagggccaagtatcacaagcatttgcggaaagattatgcaggaatagtcgaacttc
ataaatgtctgcaaatactccgagaattattgaacttgaaactgatttcttttcttcgtttgtccagaaaatgagagtcaattgatttagttgtggcata
tttggcactaataggagccaaagttacatttgattcataaacgaaagtccacaactagtcgcattaaagaaacaagaaaattaattgtgtcagttttgca
act

Border_1 (78584_LB1D)

GTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTAATGTTCATTGGAAGACTGAAATTCAGACTGAGAAA
AAGTGTAATGGATTTACTCACAATGTCAAATATGTTGAAGAGAAAGACGAAAAACGGCATATATTGAGTAATAGACCCAAGGTGGTGCTCCAACTGTGCT
TTCAGGGTTTAATATCTCCTTCATGTTTAGCAAGAAAGGTGAACCCAGAATACTAAAAACAATAAATTACCTTATTAGACAGCCTTAGATGGTAACAATA
ACACAAAGAAGTAAGAAACAGGAATGATTTAAACCCTAATAGTAAGAGGTTCTTCAAGCAATTTACCTTGCAAGAATGCTGGGGAATGCCAAAAGATATG
TGGTTGTGAGAACAGGTTGCATTCCAGCCGGGTTGATGTTAAAAGGTATGTACGGCTCCACCTCTGTTATTGGAGAACCTTCTCTGCCCAAATGGCAAAA
CATAGCATAAACACAAATATGAGTAAACCACACGTTGCATCCAAATGTACTTTAACCGTAATCTACTCCTAGCTATATTGGTAAAAGTGGCAATTATACA
ACAATCTTTTCGATAAAATAATGATGCTGAGCTACATATATCACCTCGAAGCAGAAGCAAGTTTAAAACCATAATATTGNAGTTTAATCTTCCTGCAGCC
CTCAATACACAAAG

Start of BLAST Alignment:NT 2376 / Intron 9 of 11
First 15 bp Perfect Match:NT 2376 / Intron 9 of 11
BLAST Details:
Query Sequence: 78584_LB1D 
Length: (714 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At2g31530 68415.t03575 secY family protein low similarity...  3219  3.6e-141  1
At1g11000 68414.t01123 seven transmembrane MLO family pro...   183  0.9997    1


>At2g31530 68415.t03575 secY family protein low similarity to SP|P31159
           Preprotein translocase secY subunit {Synechococcus sp}; contains
           Pfam profile PF00344: eubacterial secY protein
        Length = 3057

  Minus Strand HSPs:

 Score = 3219 (489.0 bits), Expect = 3.6e-141, P = 3.6e-141
 Identities = 649/653 (99%), Positives = 649/653 (99%), Strand = Minus / Plus

Query:   712 TTGTGTATTGAGGGCTGCAGGAAGATTAAACTNCAATATTATGGTTTTAAACTTGCTTCT 653
             |||| || |||||||||||||||||||||||| |||||||||||||||||||||||||||
Sbjct:  1726 TTGT-TACTGAGGGCTGCAGGAAGATTAAACTACAATATTATGGTTTTAAACTTGCTTCT 1784

Query:   652 GCTTCGAGGTGATATATGTAGCTCAGCATCATTATTTTATCGAAAAGATTGTTGTATAAT 593
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1785 GCTTCGAGGTGATATATGTAGCTCAGCATCATTATTTTATCGAAAAGATTGTTGTATAAT 1844

Query:   592 TGCCACTTTTACCAATATAGCTAGGAGTAGATTACGGTTAAAGTACATTTGGATGCAACG 533
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1845 TGCCACTTTTACCAATATAGCTAGGAGTAGATTACGGTTAAAGTACATTTGGATGCAACG 1904

Query:   532 TGTGGTTTACTCATATTTGTGTTTATGCTATGTTTTGCCATTTGGGCAGAGAAGGTTCTC 473
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1905 TGTGGTTTACTCATATTTGTGTTTATGCTATGTTTTGCCATTTGGGCAGAGAAGGTTCTC 1964

Query:   472 CAATAACAGAGGTGGAGCCGTACATACCTTTTAACATCAACCCGGCTGGAATGCAACCTG 413
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1965 CAATAACAGAGGTGGAGCCGTACATACCTTTTAACATCAACCCGGCTGGAATGCAACCTG 2024

Query:   412 TTCTCACAACCACATATCTTTTGGCATTCCCCAGCATTCTTGCAAGGTAAATTGCTTGAA 353
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2025 TTCTCACAACCACATATCTTTTGGCATTCCCCAGCATTCTTGCAAGGTAAATTGCTTGAA 2084

Query:   352 GAACCTCTTACTATTAGGGTTTAAATCATTCCTGTTTCTTACTTCTTTGTGTTATTGTTA 293
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2085 GAACCTCTTACTATTAGGGTTTAAATCATTCCTGTTTCTTACTTCTTTGTGTTATTGTTA 2144

Query:   292 CCATCTAAGGCTGTCTAATAAGGTAATTTATTGTTTTTAGTATTCTGGGTTCACCTTTCT 233
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2145 CCATCTAAGGCTGTCTAATAAGGTAATTTATTGTTTTTAGTATTCTGGGTTCACCTTTCT 2204

Query:   232 TGCTAAACATGAAGGAGATATTAAACCCTGAAAGCACAGTTGGAGCACCACCTTGGGTCT 173
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2205 TGCTAAACATGAAGGAGATATTAAACCCTGAAAGCACAGTTGGAGCACCACCTTGGGTCT 2264

Query:   172 ATTACTCAATATATGCCGTTTTTCGTCTTTCTCTTCAACATATTTGACATTGTGAGTAAA 113
             |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2265 ATTACTCAATATATGC-GTTTTTCGTCTTTCTCTTCAACATATTTGACATTGTGAGTAAA 2323

Query:   112 TCCATTACACTTTTTCTCAGTCTGAATTTCAGTCTTCCAATGAACATTAAACA 60
             |||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2324 TCCATTACACTTTTTCTCAGTCTGAATTTCAGTCTTCCAATGAACATTAAACA 2376


>At1g11000 68414.t01123 seven transmembrane MLO family protein / MLO-like
           protein 4 (MLO4) identical to membrane protein Mlo4 [Arabidopsis
           thaliana] gi|14091578|gb|AAK53797; similar to MLO protein
           SWISS-PROT:P93766, NCBI_gi:1877221 [Hordeum vulgare][Barley]
        Length = 4224

  Minus Strand HSPs:

 Score = 183 (33.5 bits), Expect = 8.1, P = 0.9997
 Identities = 207/369 (56%), Positives = 207/369 (56%), Strand = Minus / Plus


Border_2 (78584_LB2U)

ATCGA:TTCA:TTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCCTGA
ATTAACGGTTTCTTGTTTTGTTATGTAGGCCAACTTGCCAAAGGAAATAGCGGATTATTTAAACAAAATGGGGGCTGAGAATACCGAACATAAAGCCTGG
GAAAGCCACAATCGAGTACTTAACGAAGATACAAGCATCAACTAGGTTTTGGGGTAAGTTAAGCTGTTTCATTTTAGATATATTGCGTATATGGTACAAG
TATAATCTCTAAAAACCATTTCATTTGGTTTGATGCAGGTGGCCTTCTGTTGAGCTTTTTGGCAACAGCTTCTACTGTACTTGACCACTATCTACGCAGC
ATCAATCAAGGCTTTTCTATCGGATTTACATCTGTTTTGATCATTGTAAGTGTTCTTTCCACGTATCAATATCATTCAGTTTAGCAGTATTGATGTCGTT
GTGTT

Start of BLAST Alignment:NT 2284 / Intron 9 of 11
First 15 bp Perfect Match:NT 2388 / Intron 9 of 11
BLAST Details:
Query Sequence: 78584_LB2U 
Length: (503 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At2g31530 68415.t03575 secY family protein low similarity...  2018  6.3e-87   1
At3g62290 68416.t06463 ADP-ribosylation factor identical ...   218  0.058     1
At4g21220 68417.t02882 bacterial transferase hexapeptide ...   201  0.36      1



>At2g31530 68415.t03575 secY family protein low similarity to SP|P31159
           Preprotein translocase secY subunit {Synechococcus sp}; contains
           Pfam profile PF00344: eubacterial secY protein
        Length = 3057

  Plus Strand HSPs:

 Score = 2018 (308.8 bits), Expect = 6.3e-87, P = 6.3e-87
 Identities = 458/505 (90%), Positives = 458/505 (90%), Strand = Plus / Plus

Query:    10 TTCGGCGTTAA-TTCAGTACATTAAAAAC-GTCCGCAA-TGTGTTATTAAGTTGTCTAAG 66
             |||| | ||   ||||  | |||  | |  ||  | || |   |||  |  || ||| ||
Sbjct:  2285 TTCGTCTTTCTCTTCAACATATTTGACATTGTGAGTAAATCCATTAC-ACTTTTTCTCAG 2343

Query:    67 CGTCAATTT--GT-TTACACC-A-CAATATATCCTGA-ATTAAC--GGTTTCTTGTTTTG 118
               | |||||  || || ||   | || || | | | | ||| ||  ||||||||||||||
Sbjct:  2344 TCTGAATTTCAGTCTTCCAATGAACATTAAA-CATTACATTTACCGGGTTTCTTGTTTTG 2402

Query:   119 TTATGTAGGCCAACTTGCCAAAGGAAATAGCGGATTATTTAAACAAAATGGGGGCTGAGA 178
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
Sbjct:  2403 TTATGTAGGCCAACTTGCCAAAGGAAATAGCGGATTATTTAAACAAAATGGGGGC-GAGA 2461

Query:   179 ATACCGAACATAAAGCCTGGGAAAGCCACAATCGAGTACTTAACGAAGATACAAGCATCA 238
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2462 ATACCGAACATAAAGCCTGGGAAAGCCACAATCGAGTACTTAACGAAGATACAAGCATCA 2521

Query:   239 ACTAGGTTTTGGGGTAAGTTAAGCTGTTTCATTTTAGATATATTGCGTATATGGTACAAG 298
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2522 ACTAGGTTTTGGGGTAAGTTAAGCTGTTTCATTTTAGATATATTGCGTATATGGTACAAG 2581

Query:   299 TATAATCTCTAAAAACCATTTCATTTGGTTTGATGCAGGTGGCCTTCTGTTGAGCTTTTT 358
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2582 TATAATCTCTAAAAACCATTTCATTTGGTTTGATGCAGGTGGCCTTCTGTTGAGCTTTTT 2641

Query:   359 GGCAACAGCTTCTACTGTACTTGACCACTATCTACGCAGCATCAATCAAGGCTTTTCTAT 418
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2642 GGCAACAGCTTCTACTGTACTTGACCACTATCTACGCAGCATCAATCAAGGCTTTTCTAT 2701

Query:   419 CGGATTTACATCTGTTTTGATCATTGTAAGTGTTCTTTCCACGTATCAATATCATTCAGT 478
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2702 CGGATTTACATCTGTTTTGATCATTGTAAGTGTTCTTTCCACGTATCAATATCATTCAGT 2761

Query:   479 TTAGCAGTATTGATGTCGTTGTGTT 503
             |||||||||||||||||||||||||
Sbjct:  2762 TTAGCAGTATTGATGTCGTTGTGTT 2786


>At3g62290 68416.t06463 ADP-ribosylation factor identical to GP:166586
           ADP-ribosylation factor {Arabidopsis thaliana}; ADP-ribosylation
           factor 1 - Arabidopsis thaliana, PIR:S28875
        Length = 2093

  Plus Strand HSPs:

 Score = 218 (38.8 bits), Expect = 0.060, P = 0.058
 Identities = 148/250 (59%), Positives = 148/250 (59%), Strand = Plus / Plus