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At5g27540 SYNGENTA 80307

Genomic Sequence (from TAIR)
tgtgtttacggaaataccactagactaaacacagacaaggtaaaaaaaaaaaacagcttaccaaatttttctcctccttcacggcaaatcgtcaccgcca
cattatctcccaaactaagaatctagggttttctaagggtttttaatcttcgtccttctctccttctccagttttaggtttcaggtttatctctcgtttc
tttcatcgaatcttttgatctttaggttagttttgcttctcgttgatgttactctttccattgttatctatggttctggattcgtcatatccttcacgat
cttcatgtttctgggtttttaaaatcatgctgcgaagtttcagtaatcgtgtctcagactcgtcaatctttgtacttgattctgaattgttgagtgtttg
attgtgttgcagtttcagATGGCGAGATACGCTGCTGGTGCGGTTGATTGTCCTGGTTCTCCCAAATCTGTGCGAATCGTTGTGGTTGGTGATAAGGGCA
CTGGAAAATCAAGCTTGATTGTTGCTGCAGCCACTGATTCTTTCCCTCCCAATGTCCCTCCTGTTTTGCCTGATTACAAGTTGCCTATTGAATTCTTCCC
TGATGGTATACCCGTCACCATTGTCGACACTTCCTCGAGgtatatatccttatataagttcactgtattgctggataattactctcttaaataaagctac
tttgaaagaaaataatttctagtttagagaaaagagtgttgcttttatatacaatattgttgttgggattgcactgtgccaactgtttgtgggtttagga
tattttaagctttgccagctttgttgaggttcttgaagtatgttttgatgtttttgttcgttggttcagGCCAGAAGATAGGGACATTGTTGCTGAGGAA
TTGAAGCGCGCAGATGCAGTAGTTTTGACATATGCCTGTGATCGACCTGAGACTCTCGAACGTTTGAGTGAATACTGGCTTCCAGAGCTTCGGCGATTAG
AGgtttgattttcatctctctgttttcagtttggtcatgtcaatcttgttgtgtgtaaaaggtgtacaatatcttatctattgatcatatcattgtcata
atgctagaaggtgtgacctaaacctttgctgtttcttattaagattggcttgtgatgtttttgaacagGTAAAAATTCCTATTATCGTAGCAGGATGTAA
GCTTGACTTCAGAGACGACAATAACCAGGTCAGCCTCGAACAAGTGATGTCACCTATAATGCAGCAGTTTCGGGAGATAGAGACTTGTATCGAGTGTTCT
GCCCTGAAGCAACTCCAGgtttcatgacttctgaattcctcttgatatgtgatatttttctttgttcaaacatggcacaaatattggtccgaggtagctt
aggcatgtgcttgtcttactaagaaatttcaaggacttaagatgtgctaaatttttgcctgaaagtagatattgttgttattggtttggacttttatcag
tcatgaactggaaatgcctagtcagaaattagcattcggtggtaattacaaattatattccaggtctaaactatcatcctaatgcacaagctttcctgta
gGCACAAGAAGTTTTCTATTATGCACAAAAGACTGTCCTTCACCCAACAGGACCTCTGTTTGATCAAGATTCCCAGGCACTGAAACCCCGGTGTGTAAGG
GCATTGAAGCGTATCTTTATACTCTGTGATCATGACAGAGATGGTGCTCTTAGCGAGGCTGAGTTAAATGATTTTCAGgttcttatatttcctctttcgt
gtttcatgtgcttcgctgttcgtcattgaattggcttagccctatccttcagattttaaaaagaagcttgaagtttaattatgttatccccatgacgcac
ctaatagttgttttacaatagGTCAAGTGTTTCCATGCACCATTGCAGCCTTCTGAAATTGAAGGTGTTAAGAGGGTTGTGCAAGAAAAGTTGCCAGAAG
GTGTGAATGAAAGAGGACTGACAGTCACTGGCTTCCTATTTTTACATGCACTTTTTATTGAGAAAGGGAGGCTTGAGACTACGTGGACTGTACTCAGGAA
GTTTGGGTATAATAATGATATAAGACTTGCTGAAGAATTGCTTCCATCTGCAATATTCAAGCGAGCTCCTGATCAGgtaaatttgcagaaataacatcca
ttgttcttcattattgtatttgttggtgtttcaataatctattactggaaagagctgtatatcgagcattaaattttgtaatcttatcagaaataagaat
tttggatgcctacattactggaaaacggttatcttacagctcacattaacttgccttccttggtattgtgcagctaatgtttgtgttcattgtttttgta
gAGCTTTGAGTTGACAAATGCAGCAATCGATTTCCTGAAAGGAATGTATATGTTGTTTGATGACGACCAGgtatgtttcttttcttataaatggcgtagt
gttgagttgacaaataaatttctcagactgaatttgcactttgcagGATAACAATCTGAGACCTCAAGAGATCGAGGATCTTTTTTCAACTGCACCTGAA
AGgtaccttttttacacctccagaaggcaaagaacgtgtacttttgattatggtaatctccattatattgccatgccatactaaattgtgaatggcagTC
CATGGAAGGAAGCTCCTTATGAAGATGCTGCGGAAAAAACTGCCCTTGGAGGACTATCATTTGACGCTTTCCTGTCAATGgtaatcaatactcttttaca
tgcttaacaacataatgaagcattcttgtgcatgcattatacacatactcatccttctttattttatttttctttgattttgttgactgacatacttaaa
tttcacacaattctctctttattttcttgtaacacatccaatttatagttggattatatgtcgttttgcgattttgaatggattttactctgggccaaag
ttttgttcatgaactaattcaaagtttaattgtgtgctaatctttcaattttatccttgcagTGGTCACTGATGACACTATTAGAACCGGCTCGGAGTGT
GGAGAATTTGATTTACATTGGATTCCCGGGTGATCCATCGACTGCCATTCGTGTTACTAGGAGAAGACGTTTAGATCGCAAAAAGCAGCAATGTGAACGA
AAAGTTTTCCAGTGCTTTGTTTTTGGACCTAATAATGCTGGAAAATCTGCATTGCTGAATTGCTTTCTTGGAAGgtttgtcatgttaactcgtaccacaa
cttgttggattttcaaaatacacagaagagagtataattctatgtttataagcaaggagacgtttaattttcctctttttcagttgatctctccatctgg
tttatagttttgtttctccatgatctagGTCATATACTGATAACCAGGAATCAACTACTGATGAGCGTTATGCAGTAAATATGGTCGATGAATCGgtgag
tcagtatacttatgaaatttgtatcgttatgtttgtatgcgcgcataaatatatatgtattttagtttggttatattttgtctttgcaaagaaatcttcc
acgtgcaaaatactgaacaaaaatgacaaacttcagtgttgttttggggactgagatgggaactagctagatatctttgatgagttttctctatgggtgg
atacatgcttttttacttggaaatttcaaacaaaactgttcagagagattgaagatgttatttggatgtacatttttgggcaacatgcttcatggctact
ttctctaagtttcggtatgtaattggtcattattctgatagtggcaaatctctaaatgtagGGTGCTAAGAAAACTCTTATCATGAGGGAAATTCCAGAA
GATGGAGTTCAAGGGCTATTTTCATCCAAGGAATCCTTAGCTGCATGTGACATAGCTGTCTTCGTGTATGACAGgtatatgtcctgctgtttgattggtt
gaaatatacctctctgtatgtagtttgtacgtaataattgagttcattgttttctccagTTCTGATGAGTCGTCATGGAAGAGAGCGACCCAGTTGC
TTGTAGAAGTTGCAAATTATGGTGAGGCCACTGGGTATGAGGTCCCTTGCCTCATGGTTTCAGCAAAAGACGATCTTGATTCATCTCCTATTTCCATACA
AGAATCCACCAGGgtagggttcttattgaactcagactcaatgaatatagctcgaataattccatacatatatatttatgcacacggatcttttcgcagA
TGACACAAGATATGGGAATAGAGCCTCCGGTATCAATCAGCTCCAAATTGGGGGATTTCAACAATTTATTCCGCAAAATCTTAACTGCAGCACAACATCC
TCATTTGAGCATTCCAGAGACCGAAGCAGGCAAAAGCCGCAAACATTACAACAGGCTAATCAACCGCTCTCTTATGGCCGTTTCCAgtacgttttctttc
ctagaatcctgaatcttgactcaatttttgtcccaaacaaagaaaaacctgtaacggcttcagacccatgttgggtatacttaaatttcgatctcatgct
atgtaaacaatttatctaatttctacctcgtgatttgtaaacagTTGGTGCTGCTGCTGTGGTCGTTGGGCTGGCTGCATACCGTGTGTATGCCACAAGA
AAGAGCTCGTCTGCCTGAgaagattgagaatgaacaatccaatctcttaaggtatgaacacggaaagaggatagcaaatctcgtgtactgagtttgttta
tcgttctcaaggttttacaacttgatccagagtactgttgagtactgttcttagaagtttaatggaaatgggctttgtttgaagtaattgtggaagaaga
tctgtagcaaacacaaacatttagggacaagataaaaagagaacatagtcaatagacattgtgttgaatttcaataacaatagaactttgtctcctcgat
gttgttaatcttgctcacgtttgctctgttctcggaattaaccagcaacactttaacatcaaatgaacaaaccattaattgcacaagatctctttagagt
ttaacaaatcgacaacaatttgaagataaaaagataaaaaaaaaagcaacagaaaatcctattttgaagttttaacaggcgaccaaacaaacactttcac
aaa

Border_1 (80307_RBD)

AATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGGGAATTGAGCTCGCGGCCGCC
ATGGCATATGCTAGCATGCATAATTCCTGCAGAGCTCGCTTGAGCTTGGATCAGATTGTACGCTTAGACAACTTAACAAAAAAACTATCCTGCTGCGCGT
AATTTGAACTCAATTATTACGTACAAACTACATACAGAGAGGTATATTTCAACCAATCAAACAGCAGGACATATACCTGTCATACACGAAGACAGCTATG
TCACATGCAGCTAAGGATTCCTTGGATGAAAATAGCCCTTGAACTCCATCTTCTGGAATTTCCCTCATGATAAGAGTTTTCTTAGCACCCTACATTTAGA
GATTTGCCACTATCAGAATAATGACCAATTACATACCGAAACTTAGAGAAAGTAGCCATGAAGCATGTTGCCCAAAAATGTACATCCAAATAACATCTTC
AATCTCTCTGAACAGTTTTGTTTGAAATTTCCAAGTAAAAAAGCATGTATCCACCCATAGAGAAAACTCATCAAAGATATCTAGCTAGTTCCCATCTCAG
TCCCCAAAACAACACTGAAGTTTGTCATTTTTGTTCAGTATTTTGCACGTGGAAGATTTCTTTGCAAAGACAAAATATAACCAAACTAAAATACATATAT
NTTTATGCGCGCATACAAACATAACGATACAAATTTCATAAGTATACTGACTCACCGATTCATCGACCATATTTACTGCATAACGCTCATCAGTAGTTGA
TTCCTGGNTATCAGTATATGACCTAGATCATGGAGAACCAAACT

Start of BLAST Alignment:NT 3628 / Intron 12 of 14 (Intron 1 in 5’UTR)
First 15 bp Perfect Match:NT 3628 / Intron 12 of 14 (Intron 1 in 5’UTR)
BLAST Details:
Query Sequence: 80307_RBD 
Length: (844 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g27540 68418.t02997 GTP-binding protein-related low si...  3167  4.9e-139  1
At5g17930 68418.t01908 MA3 domain-containing protein low ...   203  0.71      1
At3g30847 68416.t03637 pseudogene, hypothetical protein        181  0.996     1


>At5g27540 68418.t02997 GTP-binding protein-related low similarity to
           Mig-2-like GTPase Mtl [Drosophila melanogaster] GI:7271872; contains
           Pfam profile PF00036: EF hand
        Length = 5030

  Minus Strand HSPs:

 Score = 3167 (481.2 bits), Expect = 4.9e-139, P = 4.9e-139
 Identities = 635/638 (99%), Positives = 635/638 (99%), Strand = Minus / Plus

Query:   842 TTTGGTTCTCCATGATCTAGGTCATATACTGATANCCAGGAATCAACTACTGATGAGCGT 783
             |||| ||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct:  3382 TTTGTTTCTCCATGATCTAGGTCATATACTGATAACCAGGAATCAACTACTGATGAGCGT 3441

Query:   782 TATGCAGTAAATATGGTCGATGAATCGGTGAGTCAGTATACTTATGAAATTTGTATCGTT 723
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3442 TATGCAGTAAATATGGTCGATGAATCGGTGAGTCAGTATACTTATGAAATTTGTATCGTT 3501

Query:   722 ATGTTTGTATGCGCGCATAAANATATATGTATTTTAGTTTGGTTATATTTTGTCTTTGCA 663
             ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct:  3502 ATGTTTGTATGCGCGCATAAATATATATGTATTTTAGTTTGGTTATATTTTGTCTTTGCA 3561

Query:   662 AAGAAATCTTCCACGTGCAAAATACTGAACAAAAATGACAAACTTCAGTGTTGTTTTGGG 603
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3562 AAGAAATCTTCCACGTGCAAAATACTGAACAAAAATGACAAACTTCAGTGTTGTTTTGGG 3621

Query:   602 GACTGAGATGGGAACTAGCTAGATATCTTTGATGAGTTTTCTCTATGGGTGGATACATGC 543
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3622 GACTGAGATGGGAACTAGCTAGATATCTTTGATGAGTTTTCTCTATGGGTGGATACATGC 3681

Query:   542 TTTTTTACTTGGAAATTTCAAACAAAACTGTTCAGAGAGATTGAAGATGTTATTTGGATG 483
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3682 TTTTTTACTTGGAAATTTCAAACAAAACTGTTCAGAGAGATTGAAGATGTTATTTGGATG 3741

Query:   482 TACATTTTTGGGCAACATGCTTCATGGCTACTTTCTCTAAGTTTCGGTATGTAATTGGTC 423
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3742 TACATTTTTGGGCAACATGCTTCATGGCTACTTTCTCTAAGTTTCGGTATGTAATTGGTC 3801

Query:   422 ATTATTCTGATAGTGGCAAATCTCTAAATGTAGGGTGCTAAGAAAACTCTTATCATGAGG 363
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3802 ATTATTCTGATAGTGGCAAATCTCTAAATGTAGGGTGCTAAGAAAACTCTTATCATGAGG 3861

Query:   362 GAAATTCCAGAAGATGGAGTTCAAGGGCTATTTTCATCCAAGGAATCCTTAGCTGCATGT 303
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3862 GAAATTCCAGAAGATGGAGTTCAAGGGCTATTTTCATCCAAGGAATCCTTAGCTGCATGT 3921

Query:   302 GACATAGCTGTCTTCGTGTATGACAGGTATATGTCCTGCTGTTTGATTGGTTGAAATATA 243
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3922 GACATAGCTGTCTTCGTGTATGACAGGTATATGTCCTGCTGTTTGATTGGTTGAAATATA 3981

Query:   242 CCTCTCTGTATGTAGTTTGTACGTAATAATTGAGTTCA 205
             ||||||||||||||||||||||||||||||||||||||
Sbjct:  3982 CCTCTCTGTATGTAGTTTGTACGTAATAATTGAGTTCA 4019


>At5g17930 68418.t01908 MA3 domain-containing protein low similarity to
           SP|Q9P6R9 Cell cycle control protein cwf22 {Schizosaccharomyces
           pombe}; contains Pfam profile PF02847: MA3 domain
        Length = 3577

  Minus Strand HSPs:

 Score = 203 (36.5 bits), Expect = 1.2, P = 0.71
 Identities = 261/476 (54%), Positives = 261/476 (54%), Strand = Minus / Plus


Border_2 (80307_LBU)

ATTACACTAGCATCTGAATTTCATAACCAATCTCGATACACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATT
AAGTTGTCTAAGCGTCAATTTGTTTACACCGCAATGTGTTTGATGAGTCGTCATGGAAGAGAGCGACCCAGTTGCTTGTAGAAGTTGCAAATTATGGTGA
GGCCACTGGGTATGAGGTCCCTTGCCTCATGGTTTCAGCAAAAGACGATCTTGATTCATCTCCTATTTCCATACAAGAATCCACCAGGGTAGGGTTCTTA
TTGAACTCAGACTCAATGAATATAGCTCGAATAATTCCATACATATATATTTATGCACACGGATCTTTTCGCAGATGACACAAGATATGGGAATAGAGCC
TCCGGTATCAATCAGCTCCAAATTGGGGGATTTCAACAATTTATTCCGCAAAATCTTAACTGCAGCACAACATCCTCATTTGAGCATTCCAGAGACCGAA
GCAGGCCAAAAGCCCGCAAACATTACAACAGGCTAATCAACCGCTCTCTTATGGCCGTTTCCAGNACGTTTTCTTTNCTAGAATCCTGAATCTTGACTCA
ATTTTTTGNCCCAACCAAAGAAAAACCTGTAACGGGCTTCAGANCCCATGGTGGGGAAACTTAAAATTCGATCTCATGCTTTGNAAACAATTTTTTTTAA
TTTCTACCTCGGGGAATTGGAAACCAGTT

Start of BLAST Alignment:NT 3585 / Intron 12 of 14 (Intron 1 in 5’UTR)
First 15 bp Perfect Match:NT 3644 / Exon 13 of 15 (Intron 1 in 5’UTR)
BLAST Details:
Query Sequence: 80307_LBU 
Length: (729 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g27540 68418.t02997 GTP-binding protein-related low si...  2683  2.0e-116  1
At3g42350 68416.t04039 hypothetical protein                   1780  4.1e-76   1
At3g05310 68416.t00524 GTP-binding protein-related low si...   709  2.4e-26   1
At3g63150 68416.t06552 GTP-binding protein-related low si...   465  3.2e-15   1


>At5g27540 68418.t02997 GTP-binding protein-related low similarity to
           Mig-2-like GTPase Mtl [Drosophila melanogaster] GI:7271872; contains
           Pfam profile PF00036: EF hand
        Length = 5030

  Plus Strand HSPs:

 Score = 2683 (408.6 bits), Expect = 2.0e-116, P = 2.0e-116
 Identities = 597/654 (91%), Positives = 597/654 (91%), Strand = Plus / Plus

Query:    78 AAAAACGTC-CGCAATGT-GTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCGCAAT 135
             | | || || |   |||| |||  || ||  |    | ||  ||| |||| | ||  |  
Sbjct:  3977 ATATACCTCTCTGTATGTAGTTTGTACGTAATAATTGAGTTCATT-GTTTTCTCC--A-- 4031

Query:   136 GTGTTTGATGAGTCGTCATGGAAGAGAGCGACCCAGTTGCTTGTAGAAGTTGCAAATTAT 195
             || | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  4032 GT-TCTGATGAGTCGTCATGGAAGAGAGCGACCCAGTTGCTTGTAGAAGTTGCAAATTAT 4090

Query:   196 GGTGAGGCCACTGGGTATGAGGTCCCTTGCCTCATGGTTTCAGCAAAAGACGATCTTGAT 255
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  4091 GGTGAGGCCACTGGGTATGAGGTCCCTTGCCTCATGGTTTCAGCAAAAGACGATCTTGAT 4150

Query:   256 TCATCTCCTATTTCCATACAAGAATCCACCAGGGTAGGGTTCTTATTGAACTCAGACTCA 315
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  4151 TCATCTCCTATTTCCATACAAGAATCCACCAGGGTAGGGTTCTTATTGAACTCAGACTCA 4210

Query:   316 ATGAATATAGCTCGAATAATTCCATACATATATATTTATGCACACGGATCTTTTCGCAGA 375
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  4211 ATGAATATAGCTCGAATAATTCCATACATATATATTTATGCACACGGATCTTTTCGCAGA 4270

Query:   376 TGACACAAGATATGGGAATAGAGCCTCCGGTATCAATCAGCTCCAAATTGGGGGATTTCA 435
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  4271 TGACACAAGATATGGGAATAGAGCCTCCGGTATCAATCAGCTCCAAATTGGGGGATTTCA 4330

Query:   436 ACAATTTATTCCGCAAAATCTTAACTGCAGCACAACATCCTCATTTGAGCATTCCAGAGA 495
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  4331 ACAATTTATTCCGCAAAATCTTAACTGCAGCACAACATCCTCATTTGAGCATTCCAGAGA 4390

Query:   496 CCGAAGCAGGCCAAAAGCCCGCAAACATTACAACAGGCTAATCAACCGCTCTCTTATGGC 555
             ||||||||||| ||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct:  4391 CCGAAGCAGGC-AAAAGCC-GCAAACATTACAACAGGCTAATCAACCGCTCTCTTATGGC 4448

Query:   556 CGTTTCCAGNACGTTTTCTTTNCTAGAATCCTGAATCTTGACTCAATTTTTTGNCCCAAC 615
             ||||||||| ||||||||||| ||||||||||||||||||||||||||||| | ||||| 
Sbjct:  4449 CGTTTCCAGTACGTTTTCTTTCCTAGAATCCTGAATCTTGACTCAATTTTT-GTCCCAAA 4507

Query:   616 CAAAGAAAAACCTGTAACGGGCTTCAGANCCCATGGTGGGGAAACTTAAAATTCGATCTC 675
             |||||||||||||||||||| ||||||| |||||| |||| | ||||||| |||||||||
Sbjct:  4508 CAAAGAAAAACCTGTAACGG-CTTCAGA-CCCATGTTGGGTATACTTAAATTTCGATCTC 4565

Query:   676 ATGCTTTGNAAACAANNNNNNNNAATTTCTACCTCGGGGAATTGGAAACCAGTT 729
             ||||| || ||||||        ||||||||||||| | | ||| |||| ||||
Sbjct:  4566 ATGCTATGTAAACAATTTATCT-AATTTCTACCTCGTG-ATTTGTAAAC-AGTT 4616


>At3g42350 68416.t04039 hypothetical protein
        Length = 2661

  Plus Strand HSPs:

 Score = 1780 (273.1 bits), Expect = 4.1e-76, P = 4.1e-76
 Identities = 412/471 (87%), Positives = 412/471 (87%), Strand = Plus / Plus