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At4g04350 SYNGENTA 81281

Genomic Sequence (from TAIR)
taaatttgaacatcgtgacttgttaagtttggtgagaggggaataaggaacgttctatatgtaaagcaatgataattaagagttaaggagttcatagttc
atgtttttctctagtttggacttgagtccaaaatccaaattaatccaaaacaatgttgagcctaaactgtcccaagactcccaacaactcaggaagctct
tgctgttttccttttaatcattgtgatattacattcttgatcgtcccacagggaaaatattttagccgttagaccaaatgataaaaaactgtaactgttg
atgggaacggcgtcgtttcgctaaccttcgttttaactgatctcaattctcaaggttcaaaagagtacacacacacttatctcaattatccactgaaaca
ttcgtctccggcgaaacaaaaccagaATGAGCTCTCATCATCAAATCCTCCAAATTCGTTCTGACCCATTTGTTCTATCACACTGTTGCCGCCACACACG
CCTCACCAGCTCTCTTACGCTTCAATCTCCTCTAAAGCAGCCCTTTTCATGTCTCCCTTTCCGTTGGCGCCGCTCTTATCGCGGCGGAGTCAGGAGCTCA
ACGACGGAGACTCATGGTAGCAAAAAAGAAGCTTTGGTGTCGGAAACGGCGACTACATCGATTGAATTGAAAAGGGTCTATCCATTTCACGAAATTGAAC
CCAAGTGGCAACGTTATTGGGAAGATAACCGAATTTTCCGGACACCAGACGATGTTGATACTTCGAAACCTAAGTTCTACGTCCTCGACATGTTTCCATA
TCCAAGgtctcttccttttaatactgattatgtagaaagttcacagatttggctattccagcttaaagaaatgaactttgtggttttgagaagtttccat
tgatttcagtagcatccttggttaggattttgaatagatgataggttttcagattttgggttttgtggaaaagatgaaatttttgtgtgtgttttgtggt
ttgttcttcagTGGAGCAGGATTACATGTGGGACATCCATTGGGTTATACAGCAACTGATATTCTAGCGAGGCTTAGGCGTATGCAAGGTTACAATGTGT
TGCATCCTATGGGTTGGGATGCTTTTGGATTGCCTGCTGAACAATATGCTATTGAGgtccattttttttctctgttctcaagtcattatgtatggatgtt
tttgttgataaaatttgtgtccttagtatttgtaatctgcattgcagACAGGAACCCATCCGAAAACTACGACATTGAAGAACATTGACCGCTTTCGATT
GCAGgtgatatatttctttcaatctagcactagcctagaaaagttactattctcttctattcatcatttccctaacagaagaaatttctcagttttgtta
tcggtttcgttgtgagaaatttatgcttgtttcatggtttaggattactgatccttgaagattatgcaaaagccattactttggactgttttgcttaggt
cttaccatgggagtaaagatctggggaccttaacaattgtagttcttttgtttgtttgcagCTCAAATCATTGGGCTTCTCATACGATTGGGACCGTGAA
CTTTCTACAACAGAGCCAGACTACTACAAATGGACACAATGGATCTTTCTTCAGCTTTATAAGAAAGGTTTGGCATATCAGgtcaacaaaatcaatgtct
agttcacatgttaaaaccgtgcatttgtgtatagaaaagttctttgaaagattgtaaactcagGCTGAAGTACCGGTCAATTGGTGCCCGGCTCTTGGTA
CTGTTTTGGCCAATGAAGAAGTGGTGGATGGTGTTAGTGAGCGTGGTGGCCACCCGGTTATAAGAAAGgtactatctattaccatctgggaatattatgt
cgtttttcccctttcgaaatctgtatttcctctgttttgaaaattctcatgaattttacagtgacattgtttattaccaagcagCCGATGAGGCAATGGA
TGCTGAAGATTACTGCGTACGCTGATCGTCTTCTAGAAGATTTGGACGAGCTTGAGTGGCCTGAAAGTATAAAGGAAATGCAAAGAAACTGGATAGGAAG
ATCCGAAGGAGCTGAGTTGAACTTTTCAATTCTTGATGGGGAAGGCCGAGAAACTGACAAAGAGATTACAGTGTACACCACGAGGCCAGATACACTTTTT
GGAGCAACgtatgcttaaaggattaattatatattcaacatctgaaatcttgatcttgattgtcttaatatgatatttgcttccattcgcagTTACATGG
TTGTGGCACCAGAGCATCAGTTGTTGTCTTACTTTGTAACCGCAGAACAGAAACAGCAAgtaagagttttttcttctttcgattattcaacttgcctttc
agattttcatgagttctctcttatggttgcagGTTGAGGAATACAAAGATTTCGCTTCGAGGAAAAGTGATCTTGAGAGAACAGAACTTCAGAAGGACAA
GACGGGCGTATTTACGGGATGTTATGCGAAAAATCCAGCTAATGGAGATGCTATTCCCATATGGGTAGCTGATTATGTTCTAGCTAGgttagtttggtct
aactgtttacattgccttgaaatgaatttgtgtgaaacagagaacgttgttatatgtgacagTTATGGAACCGGAGCAATCATGGCGGTCCCAGCTCATG
ATACTCGGGATAATGAGTTTGCGTTGAAGTATAACATTCCCATCAAGTGGGTAGTGAGAAATGAGGCAAATTCAAGTGATGATGCTAAGCAGGTTTATCC
CGGATTGGGCATTATTGAAAATTCTTCAACTTTAGAAACAGGACTTGACATAAATCAACTATCTAGCAAAGAAGCTGCTTTAAAAGTTATTGAATGGGCT
GAGAGAACTGGCAATGGAAAGAAAAAGgttcatcttcatgtctttctcatttggattatgtaaaaagtgttctacttttcttacacaaaaggtttttgg
cagGTAAATTACAAGTTGAGGGATTGGCTATTTGCACGGCAGCGTTACTGGGGTGAACCTATCCCGATTTTAATTTTGGATGAGTCTGGTGAAACTAT
TGCTATTTCAGAATCTGAACTGCCCCTCACTTTGCCTGAGTTAAATGATTTTACGCCCACTGGAACAGGGGAACCACCACTGTCAAAAGCAGTTTCATGG
gtatgttttgcggttaatatgattagtttttgggtatgcctatgccatggacattttggttatgatcgatgactttgcagGTGAACACTGTAGATCCTTC
AACAGGAAAACCTGCCAAAAGAGAAACAAGCACTATGCCACAATGGGCTGGGTCTTGCTGgtagatatttacccttcttaattcttgtatataacctcct
cattacaaaaaatgtatctcccatttgagaatccttgatgctctctcatagtgatggttctaggataggccgaattcgatcttgaaccttgttgaatata
gtacccagtgtgttgttgaaggatgttgattttaatactgagaaactgaacaaaattacatgatttttcagGTACTATCTGAGATTCATGGACCCGAAAA
ACCCTGAAGCATTAGTTGACAAGGAAAAAGAAAAgtgagagcatttcagaatgcaatcattatgtgccatctttggccagatctttaagtttttattttg
gtgtggcagATACTGGAGCCCAGTAGATGTATATGTTGGTGGTGCTGAGCATGCTGTTTTACATTTACTATACTCGAGGTTTTGGCACAAGgtgtgtatc
ctttgaaacctccctttctttaagttatattgtgatctaactacaaattttgttactgcatcattccagGTACTTTATGACATTGGTGTTGTGTCTACGA
AAGAACCATTCAAATGTGTCATAAATCAAGGAATCATCCTTGGGGAAgtaagtcgtcttttgcaccgcctttttcaaatttactcattccgttgaatgtt
ttctgcttaattgttttacttttgcagGTCCAATATACAGCTTGGAAAGATCAAGAAGGAAATTATGTATCTGCAGATACTGAAGAACGGTTAAATGAAC
ATCAACAAGTGACAATCCCGGAAGAAAAAgtatgaatcttcattctcattcactaaaactgataagctctccagtgtttttaatcttatgatctttaatg
gtctagGTTATCAAGTCCGGAGATCATTTTGTTCTGAAGGAGGATCCTAGCATTCGTCTGATTCCACGCGTTTATAAAATGAGCAAAAGTAGAGGAAATG
TCGTAAATCCTGATGATGTTGTATTAGAGTATGGTGCAGATTCTCTACGTTTGTATGAGATGTTCATGGGACCATTTAGgtatatctcaatcccaaattt
gttttttcgggttttctcgctttctcaatgtttatatctgccttcatttccttctactagGGACTCAAAAACTTGGAACACGAGTGGGATTGAAGGTGTG
CATCGTTTCTTGGCAAGAACTTGGAGACTAGTCATTGGTTTGCCTCAGTCTGATGGTTCTTTCAAAGATGGAACCTTAGTAACTGATGACGAACCAACCC
TTGAACAACTCCGAACTCTTCATAAATGCATTGCAAAGgtcatctcatccccttatgatctctttgcggtttgtttacatcttacttaaaataggtatgt
aaaatctgcagGTGACAGAGGAAATCGAGAGTACACGATTCAACACGGGAATTTCAGGGATGATGGAGTTTGTTAATGCGGCATATAAGgttagtcttag
actcttatatcggtcagctttgtgttttcactttacaagtactgatcttttctccggtttcttcttttactttgacagTGGAATAATCAACCTAGAGGAA
TCATTGAGCCTTTTGTTCTTTTGCTCTCACCTTACGCGCCTCACATGGCTGAAGAGCTTTGGTCGCGGTTAGGCCATCCAAATTCTCTGGCCTATGAAAG
CTTCCCTAAGgtagagatagtaaactgaacatataaacacaacacaggaaggtgttttgcggtactgattgttgtggtatttgtgcagGCAAACCCGGAT
TACTTAAAGAACACAACTATTGTTCTTCCAGTTCAGATAAACGGCAAGACAAGAGGCACCATAGAGGTTGAAGAAGGATGCTCTGAAGATGATGCTTTTG
TTCTAGCTTCACAGGACGACAAACTCAGGAAATATCTAGATGGACAATCAATCAAGAAGCGAATCTATGTGCCTGGGAAGATCCTCAACGTTATCCTAGA
CCGGACTAATGTCAAGGTCACTACAAAGTAGgatttagaaagaagaaacaattacaaaacctgtcaaatgaacaaaattttatctctattgagagtttcc
attatatagaaaaagattcttttagaagcaaaagatctgttcTTAGATAACATCAGAAGCAACAGTTCCTTCTTTCCATCCTCCTTGCCGAGCTCGAAGC
TCCCACATGTAAGAAAGTCTTCTCTGTTCATCTAGAGCTGCTTCTGCTTTCTCTCTTGCTAGCTCACATGTTTCCATCCCCATACTGCATTTATCAGCCT
CTTTCTGATACTGAGACGCCGCTTTCTTAGCATCTAGCAGCAACCTATCTGCACGCCATTCGTCTTCTTGAGCTTGTGCTTCTCTTAGCTTCAGCTCTTC
AGCTACCATTTCAGTAAAACTACTCTCGCTCTCTTCACTCCCTTCCTCGTGCCTCATGCAATCTTGGCAATGAAACAAGATCCAACCAAAATGCACGTGA
AATCTGCGACGTTTTAGCATtcttactaaatatataaaaagttattaccagaaaaatactcacctaagaaactgtgattgcttaatcctgcagaagaaac
attgaatcaaacgtttcaaaagttgggttttgtgaccagggaaagaagaaaggaaagacaacgataatgatgatgattaccgtcgggaatggagaggaga
ggctgagaagaacaatcacatacacaaggaggacaagaggagtggagagaatctgcaatggtctcattgagatgccaatacagagacggtcctgcaatat
atgcgacaatacacagtcccattaccactgttaccaccttcgccgtcttcttctgccttctggtcatctctctctatttctttctctctgcaaattaatg
gtgtcttctcttttgtttgaatcttagaagattctcagtaactgttctccttgaaatgtcccccaagtattggatcctttcaagacttcacgcatcatta
gat

Border_1 (81281_RB1)

CAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGGGAATTGAGCTCGCGGCCGCCATGGCATATGCTAGCATGCATAATTCCTGC
AGAGCTCGCTTGAGCTTGGATCAGATTGTCGTTTCCCGCCTTCAGTTTAAACTATCAGTGTGTAAGAAAAGTAGAACACTTTTTACATAATCCAAATGAG
AAAGACATGAAGATGAACCTTTTTCTTTCCATTGCCAGTTCTCTCAGCCCATTCAATAACTTTTAAAGCAGCTTCTTTGCTAGATAGTTGATTTATGTCA
AGTCCTGTTTCTAAAGTTGAAGAATTTTCAATAATGCCCAATCCGGGATAAACCTGCTTAGCATCATCACTTGAATTTGCCTCATTTCTCACTACCCACT
TGATGGGAATGTTATACTTCAACGCAAACTCATTATCCCGAGTATCATGAGCTGGGACCGCCATGATTGCTCCGGTTCCATAACTGTCACATATAACAAC
GTTCTCTGTTTCACACAAATTCATTTCAAGGCAATGTAAACAGTTAGACCAAACTAACCTAGCTAGAACATAATCAGCTACCCATATGGGAATAGCATCT
CCATTAGCTGGATTTTTCGCATA:ACATCCCGTAAATACGCCCGTCTTGTCCTTCTGAAGTTCTGTTCTCTCAAGATCACTTTTCCTCGAAGCGAAATCT
TTGCATTCCTCAACCTGCAACCATAAGAGAGAACTCATGAAAATCTGAAAGGCAAGTTGAATAATCGAAAGAAGAAAAAACTCTTACTTGCTGGTTCTGG
TCTGCGGTTACCAAGNAAGACAACAACTGATGCTCTGGGGCCCCACCNTGNAACCTGCGAATG

Start of BLAST Alignment:NT 2561 / Intron 9 of 19
First 15 bp Perfect Match:NT 2561 / Intron 9 of 19
BLAST Details:
Query Sequence: 81281_RB1 
Length: (862 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At4g04350 68417.t00574 leucyl-tRNA synthetase, putative /...  3420  1.8e-150  1


>At4g04350 68417.t00574 leucyl-tRNA synthetase, putative / leucine--tRNA
           ligase, putative similar to SP|P36430 Leucyl-tRNA synthetase (EC
           6.1.1.4) (Leucine--tRNA ligase) (LeuRS) {Bacillus subtilis};
           contains Pfam profile PF00133: tRNA synthetases class I (I, L, M and
           V)
        Length = 5039

  Minus Strand HSPs:

 Score = 3420 (519.2 bits), Expect = 1.8e-150, P = 1.8e-150
 Identities = 694/705 (98%), Positives = 694/705 (98%), Strand = Minus / Plus

Query:   862 CATTCGCAGGTTNCANGGTGG-GGCCCCAGAGCATCAGTTGTTGTCTTNCTTGGTAACCG 804
             ||||||||| || || ||| | ||| |||||||||||||||||||||| ||| |||||||
Sbjct:  1897 CATTCGCAG-TTACATGGTTGTGGCACCAGAGCATCAGTTGTTGTCTTACTTTGTAACCG 1955

Query:   803 CAGACCAGAACCAGCAAGTAAGAGTTTTTTCTTCTTTCGATTATTCAACTTGCCTTTCAG 744
             |||| ||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1956 CAGAACAGAAACAGCAAGTAAGAGTTTTTTCTTCTTTCGATTATTCAACTTGCCTTTCAG 2015

Query:   743 ATTTTCATGAGTTCTCTCTTATGGTTGCAGGTTGAGGAATGCAAAGATTTCGCTTCGAGG 684
             |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Sbjct:  2016 ATTTTCATGAGTTCTCTCTTATGGTTGCAGGTTGAGGAATACAAAGATTTCGCTTCGAGG 2075

Query:   683 AAAAGTGATCTTGAGAGAACAGAACTTCAGAAGGACAAGACGGGCGTATTTACGGGATGT 624
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2076 AAAAGTGATCTTGAGAGAACAGAACTTCAGAAGGACAAGACGGGCGTATTTACGGGATGT 2135

Query:   623 TATGCGAAAAATCCAGCTAATGGAGATGCTATTCCCATATGGGTAGCTGATTATGTTCTA 564
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2136 TATGCGAAAAATCCAGCTAATGGAGATGCTATTCCCATATGGGTAGCTGATTATGTTCTA 2195

Query:   563 GCTAGGTTAGTTTGGTCTAACTGTTTACATTGCCTTGAAATGAATTTGTGTGAAACAGAG 504
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2196 GCTAGGTTAGTTTGGTCTAACTGTTTACATTGCCTTGAAATGAATTTGTGTGAAACAGAG 2255

Query:   503 AACGTTGTTATATGTGACAGTTATGGAACCGGAGCAATCATGGCGGTCCCAGCTCATGAT 444
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2256 AACGTTGTTATATGTGACAGTTATGGAACCGGAGCAATCATGGCGGTCCCAGCTCATGAT 2315

Query:   443 ACTCGGGATAATGAGTTTGCGTTGAAGTATAACATTCCCATCAAGTGGGTAGTGAGAAAT 384
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2316 ACTCGGGATAATGAGTTTGCGTTGAAGTATAACATTCCCATCAAGTGGGTAGTGAGAAAT 2375

Query:   383 GAGGCAAATTCAAGTGATGATGCTAAGCAGGTTTATCCCGGATTGGGCATTATTGAAAAT 324
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2376 GAGGCAAATTCAAGTGATGATGCTAAGCAGGTTTATCCCGGATTGGGCATTATTGAAAAT 2435

Query:   323 TCTTCAACTTTAGAAACAGGACTTGACATAAATCAACTATCTAGCAAAGAAGCTGCTTTA 264
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2436 TCTTCAACTTTAGAAACAGGACTTGACATAAATCAACTATCTAGCAAAGAAGCTGCTTTA 2495

Query:   263 AAAGTTATTGAATGGGCTGAGAGAACTGGCAATGGAAAGAAAAAGGTTCATCTTCATGTC 204
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2496 AAAGTTATTGAATGGGCTGAGAGAACTGGCAATGGAAAGAAAAAGGTTCATCTTCATGTC 2555

Query:   203 TTTCTCATTTGGATTATGTAAAAAGTGTTCTACTTTTCTTACACA 159
             |||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2556 TTTCTCATTTGGATTATGTAAAAAGTGTTCTACTTTTCTTACACA 2600


Border_2 (81281_RB2)

CAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGGGAATTGAGCTCGCGGCCGCCATGGCATATGCTAGCATGCATAATTCCTGC
AGAGCTCGCTTGAGCTTGGATCAGATTGTCGTTTCCCGCCTTCAGTTTAAACTATCAGTGTTTGCACGGCAGCGTTACTGGGGTGAACCTATCCCGATTT
TAATTTTGGATGAGTCTGGTGAAACTATTGCTATTTCAGAATCTGAACTGCCCCTCACTTTGCCTGAGTTAAATGATTTTACGCCCACTGGAACAGGGGA
ACCACCACTGTCAAAAGCAGTTTCATGGGTATGTTTTGCGGTTAATATGATTAGTTTTTGGGTATGCCT

Start of BLAST Alignment:NT 2602 / Exon 10 of 20
First 15 bp Perfect Match:NT 2604 / Exon 10 of 20
BLAST Details:
Query Sequence: 81281_RB2 
Length: (369 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At4g04350 68417.t00574 leucyl-tRNA synthetase, putative /...  1046  1.5e-41   1


>At4g04350 68417.t00574 leucyl-tRNA synthetase, putative / leucine--tRNA
           ligase, putative similar to SP|P36430 Leucyl-tRNA synthetase (EC
           6.1.1.4) (Leucine--tRNA ligase) (LeuRS) {Bacillus subtilis};
           contains Pfam profile PF00133: tRNA synthetases class I (I, L, M and
           V)
        Length = 5039

  Plus Strand HSPs:

 Score = 1046 (163.0 bits), Expect = 1.5e-41, P = 1.5e-41
 Identities = 210/211 (99%), Positives = 210/211 (99%), Strand = Plus / Plus

Query:   159 TGTTTGCACGGCAGCGTTACTGGGGTGAACCTATCCCGATTTTAATTTTGGATGAGTCTG 218
             | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2641 TATTTGCACGGCAGCGTTACTGGGGTGAACCTATCCCGATTTTAATTTTGGATGAGTCTG 2700

Query:   219 GTGAAACTATTGCTATTTCAGAATCTGAACTGCCCCTCACTTTGCCTGAGTTAAATGATT 278
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2701 GTGAAACTATTGCTATTTCAGAATCTGAACTGCCCCTCACTTTGCCTGAGTTAAATGATT 2760

Query:   279 TTACGCCCACTGGAACAGGGGAACCACCACTGTCAAAAGCAGTTTCATGGGTATGTTTTG 338
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2761 TTACGCCCACTGGAACAGGGGAACCACCACTGTCAAAAGCAGTTTCATGGGTATGTTTTG 2820

Query:   339 CGGTTAATATGATTAGTTTTTGGGTATGCCT 369
             |||||||||||||||||||||||||||||||
Sbjct:  2821 CGGTTAATATGATTAGTTTTTGGGTATGCCT 2851