At2g18510 SYNGENTA 86857
Genomic Sequence (from TAIR)
ttgtctatgctttgagccaattactacgcttgcgttgaaagttaaccgtatttttttgttcttttcccccagatcatctgaactatagtctgaattgtga aaatttcatatcgtttaacaatttaggtttacagttatttgtaaaaccaattcccaaaatgattagtaaaaaaaactcaaaatctctagactctaaatag gctaaatatcttaatggatataaaagagcttaatgggcttataggcccattagagacggcaccactctaaataaacccctctttcttcaccgtccgttct aacttctagaaaaatcgccagaagtgacgaaattgaaaatctcagatttctcttaatcggcgacggagtaacgaaacaaggagaagaagaagaagaagaa aATGACGACTCGAATCGCTCCTGGTGTTGGAGCTAATCTTCTCGGCCAACACTCCGCTGAGAGGAACCAAGACGCCACTGTTTACGTCGGTGGTCTCGAT GCTCAGgttttt◄taacttttaggtttcgtgaaatgattctggattttctcatcttcctctgtgagttggtgaaattgatttaaatttcctgtttgaatc tcagCTTTCTGAAGAATTGCTTTGGGAATTGTTCGTTCAAGCTGGACCTGTTGgtaagttcatatctatatctccttaagtttcttcaatagctaatggc ttttgattagtggaacaatgatattgttcttggcctgtatcagTTAATGTCTATGTACCGAAAGATAGAGTGACAAATCTTCATCAGAACTATGGATTCA TTGAATATCGTAGTGAGGAAGATGCTGATTATgtaagtgttgctctttttcacttactgtgagaagttaatagacttttttttgtgtacaatgttgaaga gatttcgatgtttctgtgcagGCGATTAAGGTTCTTAACATGATTAAGCTCCACGGGAAGCCTATACGTGTTAACAAGgtatcgaattgtttctgtaact tacattgtttagtatcgagcggatgggttttgtgagttttgatgctgagacttgtttaatctatcttctatgagtttgctaacattttgtttgctggtta ►aatttgtattagGCATCTCAAGATAAGAAGAGCC►TGGATGTTGGTGCTAACCTTTTCATTGGAAACCTTGACCCTgtaagtatcttttgtggtagact ctgattttgatactctacaaaggtcatttaatctcttagctgaccaggttttgttttcttttatagGATGTGGATGAGAAGCTCTTGTATGATACTTTCA GTGCATTTGGTGTGATTGCTTCTAATCCTAAGgtagtattttttatttcattagattgtagttcatgattcttgatctctttatctgtctcctgtcaccg attcgatagtaaattaggattcatcggtattccactgtggagaataaatgttagaaacttttcatttcagATAATGAGAGATCCCGATACTGGAAACTCA CGAGGTTTTGGTTTCATCAGCTATGACTCCTTTGAAGCATCTGATGCTGCTATTGAGgtaattatctgcttctttcataactctcagttctatgaacttg cttacgaaaaggagcccaatatatttcacacatttaacttctccgattacctaatccaatggtagtatattcctctgatctttgtctgactattgcagTC AATGACTGGACAATATCTCAGTAATCGTCAAATCACAGTCTCTTACGCATACAAGAAAGACACCAAAGGAGAGCGCCATGGTACTCCAGCAGgttaggct ctacatactctttctcttttcttaacttatggattcagtttctctccaccattggatcaataacacattactgttgtgaattacagAGAGGCTTTTGGCA GCCACAAATCCAACTGCCCAGAAAAGCAGACCCCATACGCTATTCGCAATGGGCCCTCCATCCAGTGCTCCTCAAGTTAATGGTCTACCACGTCCGTTTG CTAACGGCAGCATGCAACCTGTCCCGATTCCCGCTCCCCGTCAACCACCACCACCTCCTCCACAAGTATATCAAACTCAGCCACCATCCTGGCCATCTCA GCCACAACAACACAGCATGGTCCCACCACCCATGCAATTCCGTCCACCTCAGGGTATGCCTCCGCCACCACCTCCTCAGTTTCTAAATCACCAACAAGGT TTCGGTGGTCCGAGGCCGCCGCCACCACCTCAAGCCATGGGAATGCACCAACATGGAGGATGGCCACCGCAACACATGCAGCAGCAAGGTGGACCACCAC AACAACAACAACCACCATACCAACACCATCACATGTCTATGCCTCCACCACCACCACACCAAGGTTGAtaataatcatcgcctcagtttcggaccaattc aactatatgtaaaccctttctttactggcaagtaatcccgaggattttccgttttagattcaatgaaacctaatagggagagagagaaagagaaagagag agggtgagagagagatcacaattaatattctatattgtcttgtctgcagagaatctcttatcttcggtttagagagagagagagcgtagaaattagtgcc aatgtgtgtctggtttttagtgatgttatttcttgtttgtgttgcagaatctctgatgccgtgtgctttaatatttaccaaaactgtgctgtaaaatctc aataattttacttctgacctttgaggttgctttttactctaaacaagaaaaagacagaggagtaatcagtgatggaacagaggtggcttgtttttcaagt tat
Border_1 (86857_AbB_U)
AAAAACCTGAGCATCGAGACCACCGACGTAAACAGTGGCGTCTTGGTTCCTCTCAGCGGAGTGTTGGCCGAGAAGATTAGCTCCAACACCAGGAGCGATT
CGAGTCGTCATTTTCTTCTTCTTCTTCTTCTCCTTGTTTCGTTACTCCGTCGCCGATTAAGAGAAATCTGAGATTTTCAATTTCGTCACTTCTGGCGATT
TTTCTAGAAGTTAGAACGGACGGTGAAGAAAGAGGGGTTTATTTAGAGTGGTGCCGTCTCTAATGGGCCTATAAGCCCATTAAGCTCTTTTATATCCATT
AAGATATTTAGCCTATTTAGAGTCTAGAGATTTTGAGTTTTTTTTACTAATCATTTTGGGAATTGGTTTTACAAATAACTGTAAACCTAAATTGTTAAAC
GATATGAAATTTTCACAATTCAGACTATAGTTCAGATGATCTGGGGGAAAAGAACAAAAAAATACGGTTAACTTTCAACGCAAGCGTAGTAATTGGCTCA
AAGCATAGACAAGTAAATTGGGAATCGTTTGTCTTCTACACGCTAGATCACAGTTTCCACAAGCCATAATATTAGCTGAAGCAAGCAAGCACAAACAAAA
ATAAGATCTTCTAAGCAACTTCTTGAGCTTTATACATCATCCTCTCTTTCTTGATCTAACACTTCTCTAGTTTGAGAAGAAGATGAAACAGCAGCTGCAT
TACCCTTGAATACAATCCCAGGTTCTCC
| Start of BLAST Alignment: | NT 111 / Intron 1 of 8 |
| First 15 bp Perfect Match: | NT 111 / Intron 1 of 8 |
BLAST Details: Query Sequence: 86857_AbB_U Length: (728 letters) Database: TIGR AGI BAC Sequences (DNA); Smallest Sum High Probability Sequences producing High-scoring Segment Pairs: Score P(N) N F24H14 47334 3432 7.4e-149 1 T13P21 48847 331 1.3e-14 2 F7C8 68083 210 0.59 1 R18I 68303 207 0.71 1 F24H14 47334 Length = 82,356 Minus Strand HSPs: Score = 3432 (521.0 bits), Expect = 7.4e-149, P = 7.4e-149 Identities = 700/729 (96%), Positives = 700/729 (96%), Strand = Minus / Plus Query: 728 GGAGAACCTGGGATTGTATTCAA-GGGTAATGCAGCTGCTGTTTCATCTTCTTCTCAAAC 670 |||||| |||||||||||||||| |||||||||||||||||||||||||||||||||||| Sbjct: 52308 GGAGAAGCTGGGATTGTATTCAAAGGGTAATGCAGCTGCTGTTTCATCTTCTTCTCAAAC 52367 Query: 669 TAGAGAAGTGTTAGATCAAGAAAGAGAGGATGATGTATAAAGCTCAAGAAGTTGCTTAGA 610 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 52368 TAGAGAAGTGTTAGATCAAGAAAGAGAGGATGATGTATAAAGCTCAAGAAGTTGCTTAGA 52427 Query: 609 AGATCTTATTTTTGTTTGTGCTTGCTTGCTTCAGCTAATATTATGGCTTGTGGAAACTGT 550 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 52428 AGATCTTATTTTTGTTTGTGCTTGCTTGCTTCAGCTAATATTATGGCTTGTGGAAACTGT 52487 Query: 549 GATCTAGCGTGTAGAAGACAAACGATTCCCAATTTACTTGTCTATGCTTTGAGCCAATTA 490 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 52488 GATCTAGCGTGTAGAAGACAAACGATTCCCAATTTACTTGTCTATGCTTTGAGCCAATTA 52547 Query: 489 CTACGCTTGCGTTGAAAGTTAACCGTATTTTTTTGTTCTTTTCCCCCAGATCATCTGAAC 430 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 52548 CTACGCTTGCGTTGAAAGTTAACCGTATTTTTTTGTTCTTTTCCCCCAGATCATCTGAAC 52607 Query: 429 TATAGTCTGAATTGTGAAAATTTCATATCGTTTAACAATTTAGGTTTACAGTTATTTGTA 370 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 52608 TATAGTCTGAATTGTGAAAATTTCATATCGTTTAACAATTTAGGTTTACAGTTATTTGTA 52667 Query: 369 AAACCAATTCCCAAAATGATTAGTNNNNNNNNCTCAAAATCTCTAGACTCTAAATAGGCT 310 |||||||||||||||||||||||| |||||||||||||||||||||||||||| Sbjct: 52668 AAACCAATTCCCAAAATGATTAGTAAAAAAAACTCAAAATCTCTAGACTCTAAATAGGCT 52727 Query: 309 AAATATCTTAATGGATATAAAAGAGCTTAATGGGCTTATAGGCCCATTAGAGACGGCACC 250 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 52728 AAATATCTTAATGGATATAAAAGAGCTTAATGGGCTTATAGGCCCATTAGAGACGGCACC 52787 Query: 249 ACTCTAAATAAACCCCTCTTTCTTCACCGTCCGTTCTAACTTCTAGAAAAATCGCCAGAA 190 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 52788 ACTCTAAATAAACCCCTCTTTCTTCACCGTCCGTTCTAACTTCTAGAAAAATCGCCAGAA 52847 Query: 189 GTGACGAAATTGAAAATCTCAGATTTCTCTTAATCGGCGACGGAGTAACGAAACAAGGNN 130 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 52848 GTGACGAAATTGAAAATCTCAGATTTCTCTTAATCGGCGACGGAGTAACGAAACAAGGAG 52907 Query: 129 NNNNNNNNNNNNNNNNNAATGACGACTCGAATCGCTCCTGGTGTTGGAGCTAATCTTCTC 70 ||||||||||||||||||||||||||||||||||||||||||| Sbjct: 52908 AAGAAGAAGAAGAAGAAAATGACGACTCGAATCGCTCCTGGTGTTGGAGCTAATCTTCTC 52967 Query: 69 GGCCAACACTCCGCTGAGAGGAACCAAGACGCCACTGTTTACGTCGGTGGTCTCGATGCT 10 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 52968 GGCCAACACTCCGCTGAGAGGAACCAAGACGCCACTGTTTACGTCGGTGGTCTCGATGCT 53027 Query: 9 CAGGTTTTT 1 ||||||||| Sbjct: 53028 CAGGTTTTT 53036 T13P21 48847 Length = 126,337 Plus Strand HSPs: Score = 331 (55.7 bits), Expect = 1.3e-14, Sum P(2) = 1.3e-14 Identities = 71/77 (92%), Positives = 71/77 (92%), Strand = Plus / Plus
Border_2 (86857_LBD)
TACACTAGCATCTGAATTTCATAACCAATCTCGATACACCAAATC:AATTCA:TTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAA
GTTGTCTAAGCGTCAATTTGTATTTGGATGTTGGTGCTAACCTTTTCATTGGAAACCTTGACCCTGTAAGTATCTTTTGTGGTAGACTCTGATTTTGATA
CTCTACAAAGGTCATTTAATCTCTTAGCCTGACCAGGTTTTGTTTTCTTTTATAGGATGTGGATGAGAAGCTCTTGTATGATACTTTCAGTGCATTTGGT
GTGATTGCTTCTAATCCTAAGGTAGTATTTTTTATTTCATTAGATTGTAGTTCATGATTCTTGATCTCTTTATCTGTCTCCTGTCACCGATTCGATAGTA
AATTAGGATTCATCGGTATTCCACTGTGGAGAATAAATGTTAGAAACTTTTCATTTCAGATAATGAGAGATCCCGATACTGGAAACTCACGAGGTTTTGG
TTTCATCAGCTATGACTCCTTTGAAGCATCTGATGCTGCTATTGAGGTAATTATCTGCTTCTTTCATAACCCTCAGTTCTATGAACTTGCTTACGAAAAG
GAGCCCAATATATTTCACACATTTAACTTCTCCGATTACCTAATCCAATGGTAGTATATTCCTCTGATCTTTGTCTGACTATTGCAGTCAATGACTGGAC
AATATCTCAGTAATCGTCAAATCACAGTCTCTTACGCATACAAGAAAGACACCAAAGGAGAGCGCCATGGTACTCCAGCAGG
| Start of BLAST Alignment: | NT 698 / Intron 4 of 8 |
| First 15 bp Perfect Match: | NT 732 / Exon 5 of 9 |
BLAST Details: Query Sequence: 86857_LBD Length: (780 letters) Database: Genes from AGI, Total Genome (DNA); Smallest Sum High Probability Sequences producing High-scoring Segment Pairs: Score P(N) N At2g18510 68415.t02044 pre-mRNA splicing factor, putative... 3273 1.6e-143 1 At2g14550 68415.t01553 pseudogene, putative pre-mRNA spli... 474 8.2e-47 3 At5g13170 68418.t01373 nodulin MtN3 family protein simila... 219 0.16 1 At4g35450 68417.t04635 ankyrin repeat family protein / AF... 212 0.32 1 >At2g18510 68415.t02044 pre-mRNA splicing factor, putative similar to SP|Q15427 Splicing factor 3B subunit 4 (Spliceosome associated protein 49) (SAP 49) (SF3b50) (Pre-mRNA splicing factor SF3b 49 kDa subunit) {Homo sapiens}; contains Pfam profile PF00076: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) Length = 2437 Plus Strand HSPs: Score = 3273 (497.1 bits), Expect = 1.6e-143, P = 1.6e-143 Identities = 677/696 (97%), Positives = 677/696 (97%), Strand = Plus / Plus Query: 86 AATGTGTTATTAAGTTGTCT-AAGCGTCAATTTGTATTTGGATGTTGGTGCTAACCTTTT 144 ||| ||| |||| | ||| ||| | || | |||||||||||||||||||||| Sbjct: 800 AATTTGT-ATTAGGCA-TCTCAAGA-T-AAGAAGAGCCTGGATGTTGGTGCTAACCTTTT 855 Query: 145 CATTGGAAACCTTGACCCTGTAAGTATCTTTTGTGGTAGACTCTGATTTTGATACTCTAC 204 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 856 CATTGGAAACCTTGACCCTGTAAGTATCTTTTGTGGTAGACTCTGATTTTGATACTCTAC 915 Query: 205 AAAGGTCATTTAATCTCTTAGCCTGACCAGGTTTTGTTTTCTTTTATAGGATGTGGATGA 264 |||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||| Sbjct: 916 AAAGGTCATTTAATCTCTTAGC-TGACCAGGTTTTGTTTTCTTTTATAGGATGTGGATGA 974 Query: 265 GAAGCTCTTGTATGATACTTTCAGTGCATTTGGTGTGATTGCTTCTAATCCTAAGGTAGT 324 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 975 GAAGCTCTTGTATGATACTTTCAGTGCATTTGGTGTGATTGCTTCTAATCCTAAGGTAGT 1034 Query: 325 ATTTTTTATTTCATTAGATTGTAGTTCATGATTCTTGATCTCTTTATCTGTCTCCTGTCA 384 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1035 ATTTTTTATTTCATTAGATTGTAGTTCATGATTCTTGATCTCTTTATCTGTCTCCTGTCA 1094 Query: 385 CCGATTCGATAGTAAATTAGGATTCATCGGTATTCCACTGTGGAGAATAAATGTTAGAAA 444 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1095 CCGATTCGATAGTAAATTAGGATTCATCGGTATTCCACTGTGGAGAATAAATGTTAGAAA 1154 Query: 445 CTTTTCATTTCAGATAATGAGAGATCCCGATACTGGAAACTCACGAGGTTTTGGTTTCAT 504 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1155 CTTTTCATTTCAGATAATGAGAGATCCCGATACTGGAAACTCACGAGGTTTTGGTTTCAT 1214 Query: 505 CAGCTATGACTCCTTTGAAGCATCTGATGCTGCTATTGAGGTAATTATCTGCTTCTTTCA 564 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1215 CAGCTATGACTCCTTTGAAGCATCTGATGCTGCTATTGAGGTAATTATCTGCTTCTTTCA 1274 Query: 565 TAACCCTCAGTTCTATGAACTTGCTTACGAAAAGGAGCCCAATATATTTCACACATTTAA 624 |||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1275 TAACTCTCAGTTCTATGAACTTGCTTACGAAAAGGAGCCCAATATATTTCACACATTTAA 1334 Query: 625 CTTCTCCGATTACCTAATCCAATGGTAGTATATTCCTCTGATCTTTGTCTGACTATTGCA 684 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1335 CTTCTCCGATTACCTAATCCAATGGTAGTATATTCCTCTGATCTTTGTCTGACTATTGCA 1394 Query: 685 GTCAATGACTGGACAATATCTCAGTAATCGTCAAATCACAGTCTCTTACGCATACAAGAA 744 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1395 GTCAATGACTGGACAATATCTCAGTAATCGTCAAATCACAGTCTCTTACGCATACAAGAA 1454 Query: 745 AGACACCAAAGGAGAGCGCCATGGTACTCCAGCAGG 780 |||||||||||||||||||||||||||||||||||| Sbjct: 1455 AGACACCAAAGGAGAGCGCCATGGTACTCCAGCAGG 1490 >At2g14550 68415.t01553 pseudogene, putative pre-mRNA splicing factor non-consensus splice donor TA at exon 5; blastp match of 67% identity and 1.1e-68 P-value to GP|28374170|gb|AAH45264.1||BC045264 Similar to splicing factor 3b, subunit 4, 49kD {Xenopus laevis} Length = 1759 Plus Strand HSPs: Score = 474 (77.2 bits), Expect = 8.2e-47, Sum P(3) = 8.2e-47 Identities = 124/150 (82%), Positives = 124/150 (82%), Strand = Plus / Plus