OSU Syngenta vbi

SeedGenes Profile: EMB 1241

Gene Information
Gene Symbol EMB 1241
Chromosome Locus At5g17710   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Chloroplast GrpE Protein
Function Details Interacts with Hsp70; Chloroplast protein maintenance
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Database Release March, 2003
Predicted Sequences
Gene:2382 nucleotides
mRNA: 1283 nucleotides
Protein:  326 amino acids
cDNA Status FLcDNA Available: AF370286
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (86%)
Model Genomes:  Synechocystis (45%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 250061_at
    
Mutant Information
Allele Symbol emb 1241-1emb 1241-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Genoplante
Mutant Line Number 14805COW5
Ecotype ColumbiaWassilewskija
Genetic Background   
ABRC Stock CS16149CS24098
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Intron 2Intron 2
Terminal Phenotype Preglobular  DetailsPreglobular  Details
Nomarski Phenotype Early Globular  ImagesGlobular  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2003March, 2004
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 24.5 %26.8 %
Chi - Square 0.21.6
Seeds Counted 10711002
Percent Top Half 48.5 %55.6 %
Chi - Square 0.23.3
Average Seed Length 370 ± 80 µm400 ± 70 µm
Average Embryo Length 15 ± 0 µm15 ± 0 µm