SeedGenes Profile: EMB 1241

Gene Information Gene Symbol EMB 1241 Chromosome Locus At5g17710   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Chloroplast GrpE Protein Function Details Interacts with Hsp70; Chloroplast protein maintenance Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2003 Predicted Sequences Gene: 2382 nucleotides mRNA: 1283 nucleotides Protein:   326 amino acids cDNA Status FLcDNA Available: AF370286 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (86%) Model Genomes:   Synechocystis (45%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 250061_at

Mutant Information Allele Symbol emb 1241-1 emb 1241-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Genoplante Mutant Line Number 14805 COW5 Ecotype Columbia Wassilewskija Genetic Background     ABRC Stock CS16149 CS24098 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 2 Intron 2 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype Early Globular  Images Globular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2003 March, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 24.5 % 26.8 % Chi - Square 0.2 1.6 Seeds Counted 1071 1002 Percent Top Half 48.5 % 55.6 % Chi - Square 0.2 3.3 Average Seed Length 370 ± 80 µm 400 ± 70 µm Average Embryo Length 15 ± 0 µm 15 ± 0 µm