OSU Syngenta vbi

SeedGenes Profile: EMB 1270

Gene Information
Gene Symbol EMB 1270
Chromosome Locus At3g18110   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function PPR Protein
Function Details Processing of organellar mRNAs
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Vernon / Salk (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:4888 nucleotides
mRNA: 4426 nucleotides
Protein:  1429 amino acids
cDNA Status EST Identified
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Vitis vinifera (67%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 258149_at
    
Mutant Information
Allele Symbol emb 1270-1emb 1270-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaVernon / Salk
Mutant Line Number 16427SALK_027183
Ecotype UncertainColumbia
Genetic Background   
ABRC Stock CS16015 
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Exon 2Exon 1
Terminal Phenotype Cotyledon  DetailsCotyledon
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2002December, 2010
Seed Color White 
Embryo Color White 
Percent Mutant Seeds 24.6 % 
Chi - Square 0.1 
Seeds Counted 994 
Percent Top Half 53.1 % 
Chi - Square 0.9 
Average Seed Length 460 ± 30 µm 
Average Embryo Length 230 ± 70 µm