SeedGenes Profile: EMB 2004

Gene Information Gene Symbol EMB 2004 Chromosome Locus At1g10510   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Uncertain Function Details Chloroplast-localized LRR protein Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2003 Predicted Sequences Gene: 4152 nucleotides mRNA: 2150 nucleotides Protein:   605 amino acids cDNA Status FLcDNA Available: AY056236 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Maize (Zea mays) (62%) Model Genomes:   Danio rerio (35%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 263212_at

Mutant Information Allele Symbol emb 2004-1 emb 2004-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 54921 20062 Ecotype Columbia Wassilewskija Genetic Background     ABRC Stock CS16164 CS16163 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Exon 3 Exon 3 to Downstream Terminal Phenotype Globular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2003 March, 2003 Seed Color White White Embryo Color White White Percent Mutant Seeds 28.3 % 24.2 % Chi - Square 6.5 0.3 Seeds Counted 1113 777 Percent Top Half 49.8 % 57.4 % Chi - Square <0.1 4.2 Average Seed Length 500 ± 40 µm 420 ± 50 µm Average Embryo Length 50 ± 20 µm 50 ± 10 µm