OSU Syngenta vbi

SeedGenes Profile: EMB 2458

Gene Information
Gene Symbol EMB 2458
Chromosome Locus At3g04340   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function FtsH Protease (AAA ATPase)
Function Details Chloroplast ATP-dependent zinc metalloprotease
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Border Recovery
Myouga / Riken (1 allele)
Database Release March, 2003
Predicted Sequences
Gene:5649 nucleotides
mRNA: 3213 nucleotides
Protein:  960 amino acids
cDNA Status FLcDNA Available: AY080629
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Medicago truncatula (75%)
Model Genomes:  Synechocystis (27%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 258585_at
    
Mutant Information
Allele Symbol emb 2458-1emb 2458-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMyouga / Riken
Mutant Line Number 77471RATM52-0252-1
Ecotype ColumbiaNossen
Genetic Background qrt 
ABRC Stock CS16181 
Mutagen Treatment T-DNATransposon
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Exon 5 
Terminal Phenotype Globular  DetailsGlobular
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2003December, 2010
Seed Color White 
Embryo Color White 
Percent Mutant Seeds 27.6 %26.1 %
Chi - Square 4.10.2
Seeds Counted 1129287
Percent Top Half 51.6 % 
Chi - Square 0.4 
Average Seed Length 520 ± 30 µm 
Average Embryo Length 90 ± 50 µm