SeedGenes Profile: EMB 2764

Gene Information Gene Symbol EMB 2764 Chromosome Locus At2g21470   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol SAE 2 (R. Vierstra) Sequence Identity Gene Class Embryo Defective Predicted Function SUMO Activating Enzyme Function Details Protein modification in the nucleus Gene Evidence Double Border Recovery Meinke; Vierstra / Salk (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release October, 2007 Predicted Sequences Gene: 3857 nucleotides mRNA: 2575 nucleotides Protein:   700 amino acids cDNA Status FLcDNA Available: AK117731 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (73%) Model Genomes:   Homo sapiens (42%) Subcellular Localization TargetP Targeted to Secretory Pathway SUBA mRNA Expression Data NASCArray Spot History for 263747_at

Mutant Information Allele Symbol emb 2764-1 emb 2764-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke; Vierstra / Salk Meinke / Salk Mutant Line Number SALK_053023 SALK_020005 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 4 Exon 11 Terminal Phenotype Preglobular  Details Cotyledon  Details Nomarski Phenotype Early Globular  Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 Seed Color White Pale Yellow Embryo Color White Pale Yellow Percent Mutant Seeds 18.8 % 27.0 % Chi - Square 18.6 2.2 Seeds Counted 909 988 Percent Top Half 55.0 % 52.1 % Chi - Square 1.7 0.5 Average Seed Length 370 ± 120 µm 530 ± 70 µm Average Embryo Length 20 ± 50 µm 350 ± 70 µm