SeedGenes Profile: EMB 2766

Gene Information Gene Symbol EMB 2766 Chromosome Locus At2g45000   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function COPII Coat Protein (Sec31) Function Details Putative chloroplast-localized vesicular trafficking protein Gene Evidence Single Border Recovery Meinke / Salk (2 alleles) Database Release October, 2007 Predicted Sequences Gene: 3625 nucleotides mRNA: 2368 nucleotides Protein:   739 amino acids cDNA Status FLcDNA Available: AY133874 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (94%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA

Mutant Information Allele Symbol emb 2766-1 emb 2766-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_071950 SALK_037337 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Intron 5 Intron 5 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 Seed Color White to Pale Yellow Pale Yellow-Green Embryo Color White to Pale Yellow Pale Yellow-Green Percent Mutant Seeds 29.0 % 26.9 % Chi - Square 5.1 2.0 Seeds Counted 597 1049 Percent Top Half 49.1 % 52.8 % Chi - Square 0.1 0.9 Average Seed Length 550 ± 70 µm 520 ± 70 µm Average Embryo Length 110 ± 80 µm 270 ± 130 µm