SeedGenes Profile: EMB 2768

Gene Information Gene Symbol EMB 2768 Chromosome Locus At3g02660   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Tyrosine tRNA Synthetase Function Details Translation in mitochondria and chloroplasts Gene Evidence Single Border Recovery Meinke / Salk (2 alleles) Database Release October, 2007 Predicted Sequences Gene: 2212 nucleotides mRNA: 1738 nucleotides Protein:   511 amino acids cDNA Status FLcDNA Available: AK221609 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (71%) Model Genomes:   Escherichia coli (43%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 258475_at

Mutant Information Allele Symbol emb 2768-1 emb 2768-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_016722 SALK_045570 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 1 Exon 1 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype Preglobular  Images Preglobular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 Seed Color White White Embryo Color White White Percent Mutant Seeds 19.9 % 21.5 % Chi - Square 14.1 6.6 Seeds Counted 1024 1005 Percent Top Half 54.9 % 71.3 % Chi - Square 2.0 39.1 Average Seed Length 180 ± 50 µm 210 ± 100 µm Average Embryo Length 15 ± 0 µm 20 ± 20 µm