SeedGenes Profile: EMB 2783

Gene Information Gene Symbol EMB 2783 Chromosome Locus At2g18290   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Anaphase-Promoting Complex (APC10) Function Details Ubiquitin protein ligase; Cell cycle control Gene Evidence Border Recovery Meinke / Salk (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 2024 nucleotides mRNA: 1028 nucleotides Protein:   192 amino acids cDNA Status FLcDNA Available: AK229538 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (91%) Model Genomes:   Homo sapiens (55%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 265336_at

Mutant Information Allele Symbol emb 2783-1 emb 2783-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_043367 SALK_123967 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Intron 4 5' UTR Terminal Phenotype Cotyledon  Details Preglobular  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color White Pale Green to Green Embryo Color White Pale Green to Green Percent Mutant Seeds 24.5 % 21.0 % Chi - Square <0.1 2.3 Seeds Counted 282 271 Percent Top Half 44.9 % 54.4 % Chi - Square 0.7 0.4 Average Seed Length 220 ± 110 µm 530 ± 50 µm Average Embryo Length 20 ± 10 µm 280 ± 120 µm