SeedGenes Profile: EMB 2785

Gene Information Gene Symbol EMB 2785 Chromosome Locus At2g31060   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function E2Fa Transcriptional Activator Function Details Cell Cycle Control Gene Evidence Border Recovery Meinke / Salk (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 5414 nucleotides mRNA: 2369 nucleotides Protein:   667 amino acids cDNA Status FLcDNA Available: AK175264 Top BLAST Hits Arabidopsis: AT5G13650.1 (43%) Plant ESTs: Medicago truncatula (84%) Model Genomes:   Escherichia coli (45%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 266471_at

Mutant Information Allele Symbol emb 2785-1 emb 2785-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_047037 SALK_135322 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 9 or 11 Intron 15 or 17 Terminal Phenotype Globular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color White White Embryo Color White White Percent Mutant Seeds 26.7 % 28.1 % Chi - Square 0.3 0.8 Seeds Counted 221 153 Percent Top Half 57.6 % 41.9 % Chi - Square 1.4 1.1 Average Seed Length 480 ± 60 µm 510 ± 40 µm Average Embryo Length 60 ± 40 µm 80 ± 60 µm