SeedGenes Profile: EMB 2786

Gene Information Gene Symbol EMB 2786 Chromosome Locus At3g17300   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Unknown Function Details Complex 1 (LYR domain) protein Gene Evidence Border Recovery Meinke / Salk (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 1660 nucleotides mRNA: 600 nucleotides Protein:   91 amino acids cDNA Status FLcDNA Available: AY059890 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (94%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 258413_at

Mutant Information Allele Symbol emb 2786-1 emb 2786-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_046721 SALK_010545 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Intron 3 Intron 3 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color White White Embryo Color White White Percent Mutant Seeds 21.7 % 21.8 % Chi - Square 1.7 1.6 Seeds Counted 286 302 Percent Top Half 59.7 % 47.0 % Chi - Square 2.3 0.2 Average Seed Length 160 ± 30 µm 230 ± 80 µm Average Embryo Length 15 ± 0 µm 15 ± 0 µm