OSU Syngenta vbi

SeedGenes Profile: EMB 2804

Gene Information
Gene Symbol EMB 2804
Chromosome Locus At3g10220   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Tubulin Folding Cofactor B
Function Details Microtubule polymerization
Gene Evidence
Border Recovery
Meinke / Salk (1 allele)
Border Recovery
Meinke / SAIL (1 allele)
Database Release December, 2010
Predicted Sequences
Gene:2475 nucleotides
mRNA: 1058 nucleotides
Protein:  243 amino acids
cDNA Status FLcDNA Available: AK176266
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (88%)
Model Genomes:  Caenorhabditis elegans (40%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
mRNA Expression Data NASCArray Spot History for 259141_at
Mutant Information
Allele Symbol emb 2804-1emb 2804-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SalkMeinke / SAIL
Mutant Line Number SALK_019471SAIL_169_D05
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock   
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence   
Location of Mutation Intron 7Intron 5
Terminal Phenotype Preglobular  DetailsPreglobular  Details
Nomarski Phenotype   Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release December, 2010December, 2010
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 19.9 %26.5 %
Chi - Square 3.30.2
Seeds Counted 241151
Percent Top Half 35.4 %45.0 %
Chi - Square 4.10.2
Average Seed Length 340 ± 70 µm340 ± 70 µm
Average Embryo Length 15 ± 0 µm15 ± 0 µm