SeedGenes Profile: EMB 2811

Gene Information Gene Symbol EMB 2811 Chromosome Locus At1g63160   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function DNA Replication Factor (RFC5) Function Details DNA replication Gene Evidence Border Recovery Meinke / GABI (1 allele) Border Recovery Meinke / SAIL (1 allele) Database Release December, 2010 Predicted Sequences Gene: 1965 nucleotides mRNA: 1263 nucleotides Protein:   333 amino acids cDNA Status FLcDNA Available: BX813429 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (94%) Model Genomes:   Homo sapiens (67%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 259690_at

Mutant Information Allele Symbol emb 2811-1 emb 2811-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / GABI Meinke / SAIL Mutant Line Number GABI_117E01 SAIL_6_C02 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 5 Exon 4 Terminal Phenotype Preglobular Preglobular Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color     Embryo Color     Percent Mutant Seeds 26.9 % 24.2 % Chi - Square 0.8 0.2 Seeds Counted 416 627 Percent Top Half     Chi - Square     Average Seed Length     Average Embryo Length