OSU Syngenta vbi

SeedGenes Profile: EMB 2813

Gene Information
Gene Symbol EMB 2813
Chromosome Locus At1g67320   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function DNA Polymerase Alpha (POLA3)
Function Details DNA replication
Gene Evidence
Border Recovery
Meinke / Salk (1 allele)
Border Recovery
Meinke / SAIL (1 allele)
Database Release December, 2010
Predicted Sequences
Gene:3581 nucleotides
mRNA: 1484 nucleotides
Protein:  449 amino acids
cDNA Status FLcDNA Available: BX815018
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Medicago truncatula (71%)
Model Genomes:  Danio rerio (41%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
mRNA Expression Data NASCArray Spot History for 264969_at
Mutant Information
Allele Symbol emb 2813-1emb 2813-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SalkMeinke / SAIL
Mutant Line Number SALK_045856SAIL_720_B06
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock   
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence   
Location of Mutation Exon 8Intron 12
Terminal Phenotype PreglobularPreglobular
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release December, 2010December, 2010
Seed Color   
Embryo Color   
Percent Mutant Seeds 13.5 %36.6 %
Chi - Square 14.8117.0
Seeds Counted 2081558
Percent Top Half   
Chi - Square   
Average Seed Length   
Average Embryo Length