SeedGenes Profile: EMB 2818

Gene Information Gene Symbol EMB 2818 Chromosome Locus At3g55010   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Phosphoribosylformylglycinamidine Cyclo-ligase (PUR5) Function Details AIR synthetase; Purine biosynthesis Gene Evidence Border Recovery Meinke / Salk (1 allele) Border Recovery Meinke / SAIL (1 allele) Database Release December, 2010 Predicted Sequences Gene: 2188 nucleotides mRNA: 1501 nucleotides Protein:   389 amino acids cDNA Status FLcDNA Available: AY060585 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (86%) Model Genomes:   Homo sapiens (47%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 251830_at

Mutant Information Allele Symbol emb 2818-1 emb 2818-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / SAIL Mutant Line Number SALK_070673 SAIL_343_A07 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Intron 3 Intron 7 Terminal Phenotype Preglobular Preglobular Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color     Embryo Color     Percent Mutant Seeds 21.0 %   Chi - Square 4.8   Seeds Counted 567   Percent Top Half     Chi - Square     Average Seed Length     Average Embryo Length