SeedGenes Profile: EMB 3113

Gene Information Gene Symbol EMB 3113 Chromosome Locus At2g33800   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Chloroplast 30S Ribosomal Protein S5 Function Details Translation in chloroplast Gene Evidence Border Recovery Myouga / Riken (1 allele) Border Recovery Meinke / Salk (1 allele) Database Release December, 2010 Predicted Sequences Gene: 1659 nucleotides mRNA: 1143 nucleotides Protein:   303 amino acids cDNA Status FLcDNA Available: BX819190 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (89%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 267435_at

Mutant Information Allele Symbol emb 3113-1 emb 3113-2 Mutant Class Embryo Defective Seed Pigment Source of Mutant Myouga / Riken Meinke / Salk Mutant Line Number RATM11-2624-1 SALK_095863 Ecotype Nossen Columbia Genetic Background     ABRC Stock     Mutagen Treatment Transposon T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 2 5' UTR Terminal Phenotype Preglobular Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color     Embryo Color     Percent Mutant Seeds 22.6 % 24.6 % Chi - Square 1.4 <0.1 Seeds Counted 455 483 Percent Top Half     Chi - Square     Average Seed Length     Average Embryo Length