SeedGenes Profile: EMB 3126

Gene Information Gene Symbol EMB 3126 Chromosome Locus At3g63490   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Chloroplast 50S Ribosomal Protein L1 Function Details Translation in chloroplast Gene Evidence Border Recovery Myouga / Riken (1 allele) Border Recovery Myouga / CSHL (1 allele) Border Recovery Myouga / JIC (1 allele) Database Release December, 2010 Predicted Sequences Gene: 2153 nucleotides mRNA: 1442 nucleotides Protein:   346 amino acids cDNA Status FLcDNA Available: AY053410 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (82%) Model Genomes:   Synechocystis (60%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 251120_at

Mutant Information Allele Symbol emb 3126-1 emb 3126-2 emb 3126-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Myouga / Riken Myouga / CSHL Myouga / JIC Mutant Line Number RATM53-3245-1 CSHL_GT6376 GT_5_101962 Ecotype Nossen Landsberg erecta Landsberg erecta Genetic Background       ABRC Stock       Mutagen Treatment Transposon Transposon Transposon Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence       Location of Mutation Intron 3 Exon 2 Exon 1 Terminal Phenotype Preglobular Preglobular Preglobular Nomarski Phenotype Globular Globular Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 December, 2010 Seed Color       Embryo Color       Percent Mutant Seeds 26.1 % 24.6 % 25.8 % Chi - Square 0.6 <0.1 0.2 Seeds Counted 881 410 555 Percent Top Half       Chi - Square       Average Seed Length       Average Embryo Length