SeedGenes Profile: EMB 3137

Gene Information Gene Symbol EMB 3137 Chromosome Locus At5g14320   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Chloroplast 30S Ribosomal Protein S13 Function Details Translation in chloroplast Gene Evidence Border Recovery Myouga / Riken (1 allele) Border Recovery Meinke / Salk (1 allele) Database Release December, 2010 Predicted Sequences Gene: 1263 nucleotides mRNA: 839 nucleotides Protein:   169 amino acids cDNA Status FLcDNA Available: BX833872 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (95%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 250190_at

Mutant Information Allele Symbol emb 3137-1 emb 3137-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Myouga / Riken Meinke / Salk Mutant Line Number RATM15-0663-1 SALK_133412 Ecotype Nossen Columbia Genetic Background     ABRC Stock     Mutagen Treatment Transposon T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Intron 1 Intron 1 Terminal Phenotype Preglobular Globular Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color     Embryo Color     Percent Mutant Seeds 24.9 % 25.9 % Chi - Square <0.1 0.4 Seeds Counted 688 725 Percent Top Half     Chi - Square     Average Seed Length     Average Embryo Length