SeedGenes Profile: EMB 3140

Gene Information Gene Symbol EMB 3140 Chromosome Locus At5g39980   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function PPR Protein Function Details Processing of organellar mRNAs Gene Evidence Border Recovery Myouga / Riken (1 allele) Border Recovery Meinke / SAIL (1 allele) Border Recovery Meinke / Salk (1 allele) Database Release December, 2010 Predicted Sequences Gene: 2361 nucleotides mRNA: 2361 nucleotides Protein:   678 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Medicago truncatula (71%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 249436_at

Mutant Information Allele Symbol emb 3140-1 emb 3140-2 emb 3140-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Myouga / Riken Meinke / SAIL Meinke / Salk Mutant Line Number RATM11-2393-1 SAIL_14_A10 SALK_129401 Ecotype Nossen Columbia Columbia Genetic Background       ABRC Stock       Mutagen Treatment Transposon T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence       Location of Mutation Exon 1 Exon 1 5' UTR Terminal Phenotype Preglobular Cotyledon Cotyledon Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 December, 2010 Seed Color       Embryo Color       Percent Mutant Seeds 24.5 % 24.0 % 25.9 % Chi - Square 0.2 0.4 0.3 Seeds Counted 1067 679 690 Percent Top Half       Chi - Square       Average Seed Length       Average Embryo Length