SeedGenes Profile: EMB 3144

Gene Information Gene Symbol EMB 3144 Chromosome Locus At5g64580   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Uncertain Function Details AAA ATPase Gene Evidence Border Recovery Myouga / Riken (1 allele) Border Recovery Meinke / SAIL (1 allele) Border Recovery Meinke / Salk (1 allele) Database Release December, 2010 Predicted Sequences Gene: 4439 nucleotides mRNA: 2823 nucleotides Protein:   855 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: AT1G50250.1 (39%) Plant ESTs: Brassica napus (95%) Model Genomes:   Synechocystis (39%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 247235_at

Mutant Information Allele Symbol emb 3144-1 emb 3144-2 emb 3144-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Myouga / Riken Meinke / SAIL Meinke / Salk Mutant Line Number RATM52-0428-1 SAIL_74_G12 SALK_113657 Ecotype Nossen Columbia Columbia Genetic Background       ABRC Stock       Mutagen Treatment Transposon T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence       Location of Mutation   Exon 8 Intron 10 Terminal Phenotype Globular Transition Cotyledon Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 December, 2010 Seed Color       Embryo Color       Percent Mutant Seeds 22.4 % 24.6 % 24.6 % Chi - Square 4.3 <0.1 <0.1 Seeds Counted 1257 820 463 Percent Top Half       Chi - Square       Average Seed Length       Average Embryo Length