SeedGenes Profile: PFI

Gene Information Gene Symbol PFI Chromosome Locus At1g71440   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Tubulin Folding Cofactor E Function Details Regulation of tubulin folding and microtubule dynamics Gene Evidence Sequenced Mutant Allele U. Mayer (2 alleles) Database Release September, 2002 Predicted Sequences Gene: 3327 nucleotides mRNA: 1820 nucleotides Protein:   531 amino acids cDNA Status FLcDNA Available: BT002403 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (56%) Model Genomes:   Danio rerio (30%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 259895_at

Mutant Information Allele Symbol pfi-UU685 pfi-UU2221 Mutant Class Embryo Defective Embryo Defective Source of Mutant U. Mayer U. Mayer Mutant Line Number     Ecotype Landsberg erecta Landsberg erecta Genetic Background     ABRC Stock     Mutagen Treatment EMS EMS Insertion Mutant No No Flanking Sequence     Location of Mutation Exon 1 Exon 1 Terminal Phenotype Preglobular  Details Preglobular Nomarski Phenotype Preglobular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2002 September, 2002 Seed Color White to Pale Yellow   Embryo Color White to Pale Yellow   Percent Mutant Seeds 41.4 %   Chi - Square 66.4   Seeds Counted 461   Percent Top Half 50.8 %   Chi - Square <0.1   Average Seed Length 430 ± 70 µm   Average Embryo Length 130 ± 80 µm