SeedGenes Profile: CYT 1

Gene Information Gene Symbol CYT 1 Chromosome Locus At2g39770   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol VTC 1 (R. Last) Sequence Identity EMB 101 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function Mannose-1-Phosphate Guanyltransferase; GDP-Mannose Pyrophosphorylase Function Details Catalyzes the production of GDP-mannose; required for N-glycosylation and vitamin C biosynthesis Gene Evidence Sequenced Mutant Allele W. Lukowitz (2 alleles) Database Release March, 2002 Predicted Sequences Gene: 2284 nucleotides mRNA: 1491 nucleotides Protein:   361 amino acids cDNA Status FLcDNA Available: AY057541 Top BLAST Hits Arabidopsis: AT3G55590.1 (84%) Plant ESTs: Brassica napus (95%) Model Genomes:   Homo sapiens (61%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 245060_at

Mutant Information Allele Symbol cyt 1-1 cyt 1-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Feldmann Meinke / Feldmann Mutant Line Number 1054 1985 Ecotype Wassilewskija Wassilewskija Genetic Background     ABRC Stock CS84607 CS84608 Mutagen Treatment T-DNA T-DNA Insertion Mutant No No Flanking Sequence     Location of Mutation Exon 4 Exon 5 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 Seed Color Pale Green Pale Yellow Embryo Color Pale Green Pale Yellow Percent Mutant Seeds 26.9 % 26.5 % Chi - Square 2.0 1.3 Seeds Counted 1102 1078 Percent Top Half 52.7 % 54.5 % Chi - Square 0.8 2.4 Average Seed Length 460 ± 40 µm 500 ± 30 µm Average Embryo Length 180 ± 30 µm 140 ± 30 µm