SeedGenes Profile: DME

Gene Information Gene Symbol DME Chromosome Locus At5g04560   Alleles in Database 1 Identity Confidence Confirmed Alias Symbol EMB 1649 (D. Meinke) Sequence Identity Gene Class 50% Defective Seeds Predicted Function DNA Glycosylase Function Details Role in gene imprinting in developing seed Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2002 Predicted Sequences Gene: 9200 nucleotides mRNA: 6475 nucleotides Protein:   1987 amino acids cDNA Status FLcDNA Available: AF521596 Top BLAST Hits Arabidopsis: AT2G36490.1 (65%) Plant ESTs: Brassica napus (84%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 250834_at

Mutant Information Allele Symbol dme-1S Mutant Class 50% Defective Seeds Source of Mutant Meinke / Syngenta Mutant Line Number 32448 Ecotype Columbia Genetic Background   ABRC Stock CS16127 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 1 Terminal Phenotype Preglobular  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release September, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 37.3 % Chi - Square 35.8 Seeds Counted 555 Percent Top Half 56.0 % Chi - Square 3.0 Average Seed Length 520 ± 60 µm Average Embryo Length 20 ± 10 µm