SeedGenes Profile: EMB 1030

Gene Information Gene Symbol EMB 1030 Chromosome Locus At5g22800   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 86 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function Alanine tRNA Synthetase Function Details Translation in mitochondria and chloroplasts Gene Evidence Border Recovery (1 allele) Single Border Recovery Meinke / Salk (1 allele) Border Recovery D. Meinke (1 allele) Database Release October, 2007 Predicted Sequences Gene: 3909 nucleotides mRNA: 3105 nucleotides Protein:   978 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: AT1G50200.1 (35%) Plant ESTs: Cotton (Gossypium) (73%) Model Genomes:   Synechocystis (56%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA

Mutant Information Allele Symbol emb 1030-1 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 2092 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation   Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release October, 2007 Seed Color White Embryo Color White Percent Mutant Seeds 28.5 % Chi - Square 7.2 Seeds Counted 1113 Percent Top Half 47.9 % Chi - Square 0.5 Average Seed Length 520 ± 60 µm Average Embryo Length 120 ± 60 µm