SeedGenes Profile: EMB 1030

Gene Information Gene Symbol EMB 1030 Chromosome Locus At5g22800   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 86 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function Alanine tRNA Synthetase Function Details Translation in mitochondria and chloroplasts Gene Evidence Border Recovery (1 allele) Single Border Recovery Meinke / Salk (1 allele) Border Recovery D. Meinke (1 allele) Database Release October, 2007 Predicted Sequences Gene: 3909 nucleotides mRNA: 3105 nucleotides Protein:   978 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: AT1G50200.1 (35%) Plant ESTs: Cotton (Gossypium) (73%) Model Genomes:   Synechocystis (56%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA

Mutant Information Allele Symbol emb 1030-2 emb 1030-1 emb 86-1 emb 86-2 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Syngenta Meinke / DuPont Meinke / DuPont Mutant Line Number SALK_100540 2092 731 2564 Ecotype Columbia Columbia Wassilewskija Wassilewskija Genetic Background         ABRC Stock         Mutagen Treatment T-DNA T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details No Flanking Sequence         Location of Mutation Exon 4   Intron 2   Terminal Phenotype Transition  Details Cotyledon  Details Transition Cotyledon Nomarski Phenotype   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test Database Release October, 2007 October, 2007 December, 2010 December, 2010 Seed Color White White     Embryo Color White White     Percent Mutant Seeds 26.0 % 28.5 % 25.8 % 25.5 % Chi - Square 0.7 7.2     Seeds Counted 1136 1113     Percent Top Half 51.0 % 47.9 % 50.4 % 45.8 % Chi - Square 0.1 0.5     Average Seed Length 520 ± 70 µm 520 ± 60 µm     Average Embryo Length 120 ± 50 µm 120 ± 60 µm