OSU Syngenta vbi

SeedGenes Profile: EMB 1030

Gene Information
Gene Symbol EMB 1030
Chromosome Locus At5g22800   tair
Alleles in Database 4   See List OR Show All
Identity Confidence Confirmed
Alias Symbol EMB 86 (D. Meinke) Complemetation Test
Gene Class Embryo Defective
Predicted Function Alanine tRNA Synthetase
Function Details Translation in mitochondria and chloroplasts
Gene Evidence
Border Recovery
Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (1 allele)
Border Recovery
D. Meinke (1 allele)
Database Release October, 2007
Predicted Sequences
Gene:3909 nucleotides
mRNA: 3105 nucleotides
Protein:  978 amino acids
cDNA Status EST Identified
Top BLAST Hits
Arabidopsis: AT1G50200.1 (35%)
Plant ESTs: Cotton (Gossypium) (73%)
Model Genomes:  Synechocystis (56%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
    
Mutant Information
Allele Symbol emb 1030-2emb 1030-1emb 86-1emb 86-2
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SalkMeinke / SyngentaMeinke / DuPontMeinke / DuPont
Mutant Line Number SALK_10054020927312564
Ecotype ColumbiaColumbiaWassilewskijaWassilewskija
Genetic Background     
ABRC Stock     
Mutagen Treatment T-DNAT-DNAT-DNAT-DNA
Insertion Mutant Yes   DetailsYes   DetailsYes   DetailsNo
Flanking Sequence     
Location of Mutation Exon 4 Intron 2 
Terminal Phenotype Transition  DetailsCotyledon  DetailsTransitionCotyledon
Nomarski Phenotype   Images
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence IdentityComplementation Test
Database Release October, 2007October, 2007December, 2010December, 2010
Seed Color WhiteWhite  
Embryo Color WhiteWhite  
Percent Mutant Seeds 26.0 %28.5 %25.8 %25.5 %
Chi - Square 0.77.2  
Seeds Counted 11361113  
Percent Top Half 51.0 %47.9 %50.4 %45.8 %
Chi - Square 0.10.5  
Average Seed Length 520 ± 70 µm520 ± 60 µm  
Average Embryo Length 120 ± 50 µm120 ± 60 µm