SeedGenes Profile: EMB 1138

Gene Information Gene Symbol EMB 1138 Chromosome Locus At5g26742   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function DEAD/DEAH Box RNA Helicase Function Details RNA binding protein; potential role in gene silencing during development Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2002 Predicted Sequences Gene: 4001 nucleotides mRNA: 2916 nucleotides Protein:   748 amino acids cDNA Status FLcDNA Available: AY102126 Top BLAST Hits Arabidopsis: AT3G22330.1 (61%) Plant ESTs: Cotton (Gossypium) (73%) Model Genomes:   Homo sapiens (39%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA

Mutant Information Allele Symbol emb 1138 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 11376 Ecotype Columbia Genetic Background   ABRC Stock CS16011 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 2 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 26.2 % Chi - Square 1.0 Seeds Counted 1205 Percent Top Half 48.7 % Chi - Square 0.2 Average Seed Length 470 ± 40 µm Average Embryo Length 80 ± 10 µm