SeedGenes Profile: EMB 1345

Gene Information Gene Symbol EMB 1345 Chromosome Locus At2g26060   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function WD Repeat Protein (Ciao1) Function Details Putative transcription factor binding protein Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release September, 2002 Predicted Sequences Gene: 3055 nucleotides mRNA: 1386 nucleotides Protein:   352 amino acids cDNA Status FLcDNA Available: AY085277 Top BLAST Hits Arabidopsis: AT4G32990.1 (69%) Plant ESTs: Brassica napus (84%) Model Genomes:   Drosophila melanogaster (48%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 266847_at

Mutant Information Allele Symbol emb 1345-1 emb 1345-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 20933 SALK_060584 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16106 CS24064 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 10 Intron 7 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype Early Globular  Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2002 March, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 22.9 % 25.3 % Chi - Square 2.7 <0.1 Seeds Counted 1135 582 Percent Top Half 50.0 % 43.5 % Chi - Square <0.1 2.4 Average Seed Length 330 ± 80 µm 320 ± 90 µm Average Embryo Length 20 ± 10 µm 15 ± 0 µm