SeedGenes Profile: EMB 1401

Gene Information Gene Symbol EMB 1401 Chromosome Locus At5g20920   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Translation Initiation Factor (EIF-2 Beta) Function Details Attachment of ribosome small subunit in translation Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2003 Predicted Sequences Gene: 2399 nucleotides mRNA: 1196 nucleotides Protein:   268 amino acids cDNA Status FLcDNA Available: AF353095 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (85%) Model Genomes:   Danio rerio (56%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 246192_at

Mutant Information Allele Symbol emb 1401 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 19520 Ecotype Wassilewskija Genetic Background   ABRC Stock CS16153 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Upstream to Exon 1 in 5' UTR Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2003 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 17.9 % Chi - Square 18.8 Seeds Counted 708 Percent Top Half 59.1 % Chi - Square 4.2 Average Seed Length 430 ± 40 µm Average Embryo Length 160 ± 80 µm