SeedGenes Profile: EMB 1427

Gene Information Gene Symbol EMB 1427 Chromosome Locus At1g80260   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Gamma Tubulin (GCP5) Function Details Microtubule nucleation Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2002 Predicted Sequences Gene: 4897 nucleotides mRNA: 3297 nucleotides Protein:   995 amino acids cDNA Status FLcDNA Available: AK228920 Top BLAST Hits Arabidopsis: AT1G20570.1 (81%) Plant ESTs: Rice (Oryza sativa) (48%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 262062_s_at

Mutant Information Allele Symbol emb 1427-2 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 65508 Ecotype Columbia Genetic Background qrt ABRC Stock CS16144 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 4 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release September, 2002 Seed Color Pale Yellow-Green Embryo Color Pale Yellow-Green Percent Mutant Seeds 20.8 % Chi - Square 11.1 Seeds Counted 1156 Percent Top Half 52.5 % Chi - Square 0.6 Average Seed Length 560 ± 130 µm Average Embryo Length 210 ± 150 µm