OSU Syngenta vbi

SeedGenes Profile: EMB 1738

Gene Information
Gene Symbol EMB 1738
Chromosome Locus At1g11680   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol CYP51A 2 (K. Feldmann) Sequence Identity
Gene Class Embryo Defective
Predicted Function Obtusifoliol 14-Alpha Demethylase (CYP51)
Function Details Cytochrome P450 monooxygenase; Catalyzes sterol biosynthestic step upstream of FACKEL
Gene Evidence
Molecular Complementation
K. Feldmann (1 allele)
Border Recovery
K. Feldmann (1 allele)
Double Border Recovery
Meinke / Syngenta (1 allele)
Database Release September, 2002
Predicted Sequences
Gene:2254 nucleotides
mRNA: 1832 nucleotides
Protein:  488 amino acids
cDNA Status FLcDNA Available: AF410338
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (93%)
Model Genomes:  Homo sapiens (38%)
Subcellular Localization TargetP Targeted to Secretory Pathway
SUBA
mRNA Expression Data NASCArray Spot History for 262820_at
    
Mutant Information
Allele Symbol emb 1738cyp51a 2-3
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaK. Feldmann
Mutant Line Number 37351 
Ecotype ColumbiaWassilewskija
Genetic Background   
ABRC Stock CS16138 
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Exon 1 in 5' UTR to Exon 2Exon 2
Terminal Phenotype Cotyledon  DetailsCotyledon
Nomarski Phenotype   Images
Allelism Evidence Reference AlleleSequence Identity
Database Release September, 2002October, 2007
Seed Color White 
Embryo Color White 
Percent Mutant Seeds 28.3 % 
Chi - Square 6.2 
Seeds Counted 1067 
Percent Top Half 54.3 % 
Chi - Square 2.2 
Average Seed Length 580 ± 50 µm 
Average Embryo Length 150 ± 60 µm