SeedGenes Profile: EMB 1738

Gene Information Gene Symbol EMB 1738 Chromosome Locus At1g11680   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol CYP51A 2 (K. Feldmann) Sequence Identity Gene Class Embryo Defective Predicted Function Obtusifoliol 14-Alpha Demethylase (CYP51) Function Details Cytochrome P450 monooxygenase; Catalyzes sterol biosynthestic step upstream of FACKEL Gene Evidence Molecular Complementation K. Feldmann (1 allele) Border Recovery K. Feldmann (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2002 Predicted Sequences Gene: 2254 nucleotides mRNA: 1832 nucleotides Protein:   488 amino acids cDNA Status FLcDNA Available: AF410338 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (93%) Model Genomes:   Homo sapiens (38%) Subcellular Localization TargetP Targeted to Secretory Pathway SUBA mRNA Expression Data NASCArray Spot History for 262820_at

Mutant Information Allele Symbol emb 1738 cyp51a 2-3 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta K. Feldmann Mutant Line Number 37351   Ecotype Columbia Wassilewskija Genetic Background     ABRC Stock CS16138   Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 1 in 5' UTR to Exon 2 Exon 2 Terminal Phenotype Cotyledon  Details Cotyledon Nomarski Phenotype   Images Allelism Evidence Reference Allele Sequence Identity Database Release September, 2002 October, 2007 Seed Color White   Embryo Color White   Percent Mutant Seeds 28.3 %   Chi - Square 6.2   Seeds Counted 1067   Percent Top Half 54.3 %   Chi - Square 2.2   Average Seed Length 580 ± 50 µm   Average Embryo Length 150 ± 60 µm