SeedGenes Profile: EMB 2191

Gene Information Gene Symbol EMB 2191 Chromosome Locus At1g48175   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol TAD 2 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function tRNA Adenosine Deaminase Function Details Catalytic (TAD2) subunit; Modification of wobble position Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2003 Predicted Sequences Gene: 1825 nucleotides mRNA: 890 nucleotides Protein:   182 amino acids cDNA Status FLcDNA Available: BT014757 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (87%) Model Genomes:   Homo sapiens (50%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA

Mutant Information Allele Symbol emb 2191-2 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 92954 Ecotype Columbia Genetic Background   ABRC Stock CS16171 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Intron 1 to Intron 2 Terminal Phenotype Preglobular  Details Nomarski Phenotype Early Globular  Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2003 Seed Color White Embryo Color White Percent Mutant Seeds 24.2 % Chi - Square 0.4 Seeds Counted 1221 Percent Top Half 49.5 % Chi - Square <0.1 Average Seed Length 400 ± 70 µm Average Embryo Length 15 ± 0 µm