OSU Syngenta vbi

SeedGenes Profile: EMB 2191

Gene Information
Gene Symbol EMB 2191
Chromosome Locus At1g48175   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol TAD 2 (D. Meinke) Alternate Name
Gene Class Embryo Defective
Predicted Function tRNA Adenosine Deaminase
Function Details Catalytic (TAD2) subunit; Modification of wobble position
Gene Evidence
Double Border Recovery
Meinke / Syngenta (2 alleles)
Database Release March, 2003
Predicted Sequences
Gene:1825 nucleotides
mRNA: 890 nucleotides
Protein:  182 amino acids
cDNA Status FLcDNA Available: BT014757
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (87%)
Model Genomes:  Homo sapiens (50%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
    
Mutant Information
Allele Symbol emb 2191-1emb 2191-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Syngenta
Mutant Line Number 6888092954
Ecotype ColumbiaColumbia
Genetic Background qrt 
ABRC Stock CS16170CS16171
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence   
Location of Mutation Intron 4Intron 1 to Intron 2
Terminal Phenotype Preglobular  DetailsPreglobular  Details
Nomarski Phenotype Early Globular  ImagesEarly Globular  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2003March, 2003
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 23.2 %24.2 %
Chi - Square 1.40.4
Seeds Counted 7941221
Percent Top Half 54.9 %49.5 %
Chi - Square 2.0<0.1
Average Seed Length 370 ± 60 µm400 ± 70 µm
Average Embryo Length 15 ± 0 µm15 ± 0 µm