SeedGenes Profile: EMB 2220

Gene Information Gene Symbol EMB 2220 Chromosome Locus At5g12840   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class 50% Defective Seeds Predicted Function CCAAT Box Transcription Factor (Hap2A) Function Details Regulation of transcription Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2002 Predicted Sequences Gene: 2695 nucleotides mRNA: 1275 nucleotides Protein:   272 amino acids cDNA Status FLcDNA Available: Y13720 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (77%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 250320_at

Mutant Information Allele Symbol emb 2220 Mutant Class 50% Defective Seeds Source of Mutant Meinke / Syngenta Mutant Line Number 70913 Ecotype Columbia Genetic Background qrt ABRC Stock CS16131 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 1 in 5' UTR Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release September, 2002 Seed Color White to Pale Yellow Embryo Color White to Pale Yellow Percent Mutant Seeds 48.1 % Chi - Square 0.8 Seeds Counted 559 Percent Top Half 48.7 % Chi - Square 0.2 Average Seed Length 550 ± 60 µm Average Embryo Length 70 ± 80 µm