SeedGenes Profile: EMB 2247

Gene Information Gene Symbol EMB 2247 Chromosome Locus At5g16715   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Valine tRNA Synthetase Function Details Translation in chloroplast Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Database Release September, 2003 Predicted Sequences Gene: 8168 nucleotides mRNA: 3204 nucleotides Protein:   970 amino acids cDNA Status FLcDNA Available: BX830792 Top BLAST Hits Arabidopsis: AT1G14610.1 (39%) Plant ESTs: Rice (Oryza sativa) (77%) Model Genomes:   Synechocystis (43%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 246509_at

Mutant Information Allele Symbol emb 2247-2 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 67816 Ecotype Columbia Genetic Background qrt ABRC Stock CS16213 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 4 to Intron 4 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release September, 2003 Seed Color White Embryo Color White Percent Mutant Seeds 27.7 % Chi - Square 4.1 Seeds Counted 1084 Percent Top Half 51.7 % Chi - Square 0.3 Average Seed Length 520 ± 60 µm Average Embryo Length 70 ± 30 µm