SeedGenes Profile: EMB 2271

Gene Information Gene Symbol EMB 2271 Chromosome Locus At4g21130   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function WD-40 Repeat; U3 snoRNP Associated Protein Function Details Ribosome biogenesis; pre-rRNA processing Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2003 Predicted Sequences Gene: 2004 nucleotides mRNA: 1465 nucleotides Protein:   479 amino acids cDNA Status FLcDNA Available: AY874865 Top BLAST Hits Arabidopsis: AT4G05410.1 (62%) Plant ESTs: Vitis vinifera (51%) Model Genomes:   Danio rerio (37%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 254454_at

Mutant Information Allele Symbol emb 2271 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 74963 Ecotype Columbia Genetic Background qrt ABRC Stock CS16173 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Intron 5 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2003 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 26.8 % Chi - Square 1.5 Seeds Counted 865 Percent Top Half 45.7 % Chi - Square 1.7 Average Seed Length 530 ± 50 µm Average Embryo Length 240 ± 50 µm