SeedGenes Profile: EMB 2369

Gene Information Gene Symbol EMB 2369 Chromosome Locus At4g04350   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Leucyl tRNA Synthetase Function Details Translation in chloroplast Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Genoplante (1 allele) Database Release March, 2004 Predicted Sequences Gene: 5040 nucleotides mRNA: 3060 nucleotides Protein:   973 amino acids cDNA Status FLcDNA Available: AY079159 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (79%) Model Genomes:   Synechocystis (45%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 255328_at

Mutant Information Allele Symbol emb 2369-2 Mutant Class Embryo Defective Source of Mutant Meinke / Genoplante Mutant Line Number EVM17 Ecotype Wassilewskija Genetic Background   ABRC Stock CS24099 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 6 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2004 Seed Color White Embryo Color White Percent Mutant Seeds 25.8 % Chi - Square 0.4 Seeds Counted 1159 Percent Top Half 52.2 % Chi - Square 0.6 Average Seed Length 430 ± 60 µm Average Embryo Length 70 ± 40 µm