OSU Syngenta vbi

SeedGenes Profile: EMB 2369

Gene Information
Gene Symbol EMB 2369
Chromosome Locus At4g04350   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Leucyl tRNA Synthetase
Function Details Translation in chloroplast
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Genoplante (1 allele)
Database Release March, 2004
Predicted Sequences
Gene:5040 nucleotides
mRNA: 3060 nucleotides
Protein:  973 amino acids
cDNA Status FLcDNA Available: AY079159
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Vitis vinifera (79%)
Model Genomes:  Synechocystis (45%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 255328_at
Mutant Information
Allele Symbol emb 2369-2
Mutant Class Embryo Defective
Source of Mutant Meinke / Genoplante
Mutant Line Number EVM17
Ecotype Wassilewskija
Genetic Background  
ABRC Stock CS24099
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Exon 6
Terminal Phenotype Globular  Details
Nomarski Phenotype   Images
Allelism Evidence Complementation Test; Sequence Identity
Database Release March, 2004
Seed Color White
Embryo Color White
Percent Mutant Seeds 25.8 %
Chi - Square 0.4
Seeds Counted 1159
Percent Top Half 52.2 %
Chi - Square 0.6
Average Seed Length 430 ± 60 µm
Average Embryo Length 70 ± 40 µm