OSU Syngenta vbi

SeedGenes Profile: EMB 2369

Gene Information
Gene Symbol EMB 2369
Chromosome Locus At4g04350   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Leucyl tRNA Synthetase
Function Details Translation in chloroplast
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Genoplante (1 allele)
Database Release March, 2004
Predicted Sequences
Gene:5040 nucleotides
mRNA: 3060 nucleotides
Protein:  973 amino acids
cDNA Status FLcDNA Available: AY079159
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Vitis vinifera (79%)
Model Genomes:  Synechocystis (45%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 255328_at
Mutant Information
Allele Symbol emb 2369-1emb 2369-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Genoplante
Mutant Line Number 81281EVM17
Ecotype ColumbiaWassilewskija
Genetic Background qrt 
ABRC Stock CS24052CS24099
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Intron 9 to Exon 10Exon 6
Terminal Phenotype Globular  DetailsGlobular  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2004March, 2004
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 24.8 %25.8 %
Chi - Square <0.10.4
Seeds Counted 10641159
Percent Top Half 45.8 %52.2 %
Chi - Square 1.80.6
Average Seed Length 530 ± 70 µm430 ± 60 µm
Average Embryo Length 80 ± 30 µm70 ± 40 µm