OSU Syngenta vbi

SeedGenes Profile: EMB 2411

Gene Information
Gene Symbol EMB 2411
Chromosome Locus At1g08840   tair
Alleles in Database 4   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Helicase/Nuclease (Dna2)
Function Details DNA replication; Okazaki fragment metabolism
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (2 alleles)
Database Release March, 2003
Predicted Sequences
Gene:8791 nucleotides
mRNA: 3891 nucleotides
Protein:  1296 amino acids
cDNA Status Unknown
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Rice (Oryza sativa) (54%)
Model Genomes:  Neurospora crassa (34%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 264801_at
Mutant Information
Allele Symbol emb 2411-2
Mutant Class Embryo Defective
Source of Mutant Meinke / Genoplante
Mutant Line Number DYE165
Ecotype Wassilewskija
Genetic Background  
ABRC Stock CS24093
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Exon 20
Terminal Phenotype Preglobular  Details
Nomarski Phenotype Globular  Images
Allelism Evidence Complementation Test; Sequence Identity
Database Release March, 2004
Seed Color Pale Yellow
Embryo Color Pale Yellow
Percent Mutant Seeds 28.3 %
Chi - Square 5.1
Seeds Counted 876
Percent Top Half 58.5 %
Chi - Square 7.1
Average Seed Length 490 ± 70 µm
Average Embryo Length 20 ± 0 µm