OSU Syngenta vbi

SeedGenes Profile: EMB 2411

Gene Information
Gene Symbol EMB 2411
Chromosome Locus At1g08840   tair
Alleles in Database 4   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Helicase/Nuclease (Dna2)
Function Details DNA replication; Okazaki fragment metabolism
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (2 alleles)
Database Release March, 2003
Predicted Sequences
Gene:8791 nucleotides
mRNA: 3891 nucleotides
Protein:  1296 amino acids
cDNA Status Unknown
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Rice (Oryza sativa) (54%)
Model Genomes:  Neurospora crassa (34%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 264801_at
Mutant Information
Allele Symbol emb 2411-1emb 2411-2emb 2411-3emb 2411-4
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / GenoplanteMeinke / SalkMeinke / Salk
Mutant Line Number 110058DYE165SALK_020865SALK_022652
Ecotype ColumbiaWassilewskijaColumbiaColumbia
Genetic Background     
ABRC Stock CS24053CS24093CS24084CS24085
Mutagen Treatment T-DNAT-DNAT-DNAT-DNA
Insertion Mutant Yes   DetailsYes   DetailsYes   DetailsYes   Details
Flanking Sequence Details  (Help)   
Location of Mutation Exon 10 to Intron 10Exon 20Intron 7Exon 18
Terminal Phenotype Globular  DetailsPreglobular  DetailsGlobular  DetailsGlobular  Details
Nomarski Phenotype   ImagesGlobular  Images  Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence IdentityComplementation Test; Sequence Identity
Database Release March, 2004March, 2004March, 2004March, 2004
Seed Color Pale YellowPale YellowPale YellowPale Yellow
Embryo Color Pale YellowPale YellowPale YellowPale Yellow
Percent Mutant Seeds 27.1 %28.3 %27.7 %25.4 %
Chi - Square
Seeds Counted 86387612591233
Percent Top Half 50.4 %58.5 %51.2 %50.5 %
Chi - Square <<0.1
Average Seed Length 530 ± 60 µm490 ± 70 µm500 ± 60 µm500 ± 60 µm
Average Embryo Length 30 ± 20 µm20 ± 0 µm30 ± 20 µm30 ± 10 µm