Aug 17, 2018 4:41 PM
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Database Query
SeedGenes Profile: EMB 2411
Gene Information
Gene Symbol
EMB 2411
Chromosome Locus
At1g08840
Alleles in Database
4
See List
OR
Show All
Identity Confidence
Confirmed
Alias Symbol
Gene Class
Embryo Defective
Predicted Function
Helicase/Nuclease (Dna2)
Function Details
DNA replication; Okazaki fragment metabolism
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (2 alleles)
Database Release
March, 2003
Predicted Sequences
Gene:
8791 nucleotides
mRNA:
3891 nucleotides
Protein:
1296 amino acids
cDNA Status
Unknown
Top BLAST Hits
Arabidopsis
:
Only self hit
Plant ESTs:
Rice (Oryza sativa) (54%)
Model Genomes:
Neurospora crassa (34%)
Subcellular Localization
TargetP
Targeted to Chloroplasts
SUBA
mRNA Expression Data
NASCArray Spot History for
264801_at
Mutant Information
Allele Symbol
emb 2411-1
emb 2411-2
emb 2411-3
emb 2411-4
Mutant Class
Embryo Defective
Embryo Defective
Embryo Defective
Embryo Defective
Source of Mutant
Meinke / Syngenta
Meinke / Genoplante
Meinke / Salk
Meinke / Salk
Mutant Line Number
110058
DYE165
SALK_020865
SALK_022652
Ecotype
Columbia
Wassilewskija
Columbia
Columbia
Genetic Background
ABRC Stock
CS24053
CS24093
CS24084
CS24085
Mutagen Treatment
T-DNA
T-DNA
T-DNA
T-DNA
Insertion Mutant
Yes
Details
Yes
Details
Yes
Details
Yes
Details
Flanking Sequence
Details
(Help)
Location of Mutation
Exon 10 to Intron 10
Exon 20
Intron 7
Exon 18
Terminal Phenotype
Globular
Details
Preglobular
Details
Globular
Details
Globular
Details
Nomarski Phenotype
Images
Globular
Images
Images
Images
Allelism Evidence
Reference Allele
Complementation Test; Sequence Identity
Complementation Test; Sequence Identity
Complementation Test; Sequence Identity
Database Release
March, 2004
March, 2004
March, 2004
March, 2004
Seed Color
Pale Yellow
Pale Yellow
Pale Yellow
Pale Yellow
Embryo Color
Pale Yellow
Pale Yellow
Pale Yellow
Pale Yellow
Percent Mutant Seeds
27.1 %
28.3 %
27.7 %
25.4 %
Chi - Square
2.1
5.1
4.9
0.7
Seeds Counted
863
876
1259
1233
Percent Top Half
50.4 %
58.5 %
51.2 %
50.5 %
Chi - Square
<0.1
7.1
0.2
<0.1
Average Seed Length
530 ± 60 µm
490 ± 70 µm
500 ± 60 µm
500 ± 60 µm
Average Embryo Length
30 ± 20 µm
20 ± 0 µm
30 ± 20 µm
30 ± 10 µm