SeedGenes Profile: EMB 2411

Gene Information Gene Symbol EMB 2411 Chromosome Locus At1g08840   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Helicase/Nuclease (Dna2) Function Details DNA replication; Okazaki fragment metabolism Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (2 alleles) Database Release March, 2003 Predicted Sequences Gene: 8791 nucleotides mRNA: 3891 nucleotides Protein:   1296 amino acids cDNA Status Unknown Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (54%) Model Genomes:   Neurospora crassa (34%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 264801_at

Mutant Information Allele Symbol emb 2411-3 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_020865 Ecotype Columbia Genetic Background   ABRC Stock CS24084 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Intron 7 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2004 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 27.7 % Chi - Square 4.9 Seeds Counted 1259 Percent Top Half 51.2 % Chi - Square 0.2 Average Seed Length 500 ± 60 µm Average Embryo Length 30 ± 20 µm