SeedGenes Profile: EMB 2411

Gene Information Gene Symbol EMB 2411 Chromosome Locus At1g08840   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Helicase/Nuclease (Dna2) Function Details DNA replication; Okazaki fragment metabolism Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (2 alleles) Database Release March, 2003 Predicted Sequences Gene: 8791 nucleotides mRNA: 3891 nucleotides Protein:   1296 amino acids cDNA Status Unknown Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (54%) Model Genomes:   Neurospora crassa (34%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 264801_at

Mutant Information Allele Symbol emb 2411-4 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_022652 Ecotype Columbia Genetic Background   ABRC Stock CS24085 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 18 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2004 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 25.4 % Chi - Square 0.7 Seeds Counted 1233 Percent Top Half 50.5 % Chi - Square <0.1 Average Seed Length 500 ± 60 µm Average Embryo Length 30 ± 10 µm