SeedGenes Profile: EMB 2448

Gene Information Gene Symbol EMB 2448 Chromosome Locus At4g11150   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol VHAE 1 (K. Schumacher) Sequence Identity TUF (U. Mayer) Sequence Identity Gene Class Embryo Defective Predicted Function Vacuolar H+ ATPase (VHA-E1) Function Details Vacuolar proton pump component Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Sequenced Mutant Allele U. Mayer (1 allele) Molecular Complementation U. Mayer (1 allele) Border Recovery Mayer / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 1998 nucleotides mRNA: 1085 nucleotides Protein:   230 amino acids cDNA Status FLcDNA Available: AY065119 Top BLAST Hits Arabidopsis: AT1G64200.1 (80%) Plant ESTs: Brassica napus (94%) Model Genomes:   Drosophila melanogaster (44%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 254903_at

Mutant Information Allele Symbol tuf-2 Mutant Class Embryo Defective Source of Mutant Mayer / Salk Mutant Line Number SALK_087937 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Intron 1 Terminal Phenotype Globular Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release October, 2007 Seed Color   Embryo Color   Percent Mutant Seeds   Chi - Square   Seeds Counted   Percent Top Half   Chi - Square   Average Seed Length   Average Embryo Length