SeedGenes Profile: EMB 2448

Gene Information Gene Symbol EMB 2448 Chromosome Locus At4g11150   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol VHAE 1 (K. Schumacher) Sequence Identity TUF (U. Mayer) Sequence Identity Gene Class Embryo Defective Predicted Function Vacuolar H+ ATPase (VHA-E1) Function Details Vacuolar proton pump component Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Sequenced Mutant Allele U. Mayer (1 allele) Molecular Complementation U. Mayer (1 allele) Border Recovery Mayer / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 1998 nucleotides mRNA: 1085 nucleotides Protein:   230 amino acids cDNA Status FLcDNA Available: AY065119 Top BLAST Hits Arabidopsis: AT1G64200.1 (80%) Plant ESTs: Brassica napus (94%) Model Genomes:   Drosophila melanogaster (44%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 254903_at

Mutant Information Allele Symbol emb 2448 tuf-1 tuf-2 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta U. Mayer Mayer / Salk Mutant Line Number 88248 UU2573 SALK_087937 Ecotype Columbia Landsberg erecta Columbia Genetic Background       ABRC Stock CS16180     Mutagen Treatment T-DNA EMS T-DNA Insertion Mutant Yes   Details No Yes   Details Flanking Sequence Details  (Help)     Location of Mutation Intron 3 to Intron 4 Exon 4 Intron 1 Terminal Phenotype Preglobular  Details Globular Globular Nomarski Phenotype   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2003 October, 2007 October, 2007 Seed Color White to Pale Yellow     Embryo Color White to Pale Yellow     Percent Mutant Seeds 23.5 %     Chi - Square 1.2     Seeds Counted 966     Percent Top Half 51.1 %     Chi - Square 0.1     Average Seed Length 470 ± 40 µm     Average Embryo Length 15 ± 0 µm